DNA methylation changes in promoter region ofCDKN2Agene in workers exposed in construction environment

Autor(es)

Silva, Isana Rodrigues ^[1] ; Francisco, Luiza Flavia Veiga ^[1] ; Bernardo, Cassia ^[1] ; Oliveira, Marco Antonio ^[2] ; Barbosa, Jr., Fernando ^[3] ; Silveira, Henrique Cesar Santejo ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos, SP - Brazil

^[2] Barretos Canc Hosp, Ctr Res Support NAP, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Sao Paulo - Brazil

^[4] Univ Cuiaba, BR-78008000 Cuiaba, Mato Grosso - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMARKERS; v. 25, n. 7, p. 594-602, OCT 2 2020.
• Citações Web of Science: 1

Resumo

Purpose Construction workers are exposed to a mixture of substances in the workplace considered carcinogenic. This study aimed to characterise gene-specific changes in DNA methylation over the workweek in this population as this type of environmental exposure has not been studied extensively. Materials and methods We evaluated their DNA methylation in 4 gene-promoter regions (CDKN2A, RASSF1A, MLH1andAPC) and 2 repeat elements (ALUandLINE-1) in blood samples obtained on the first and fifth day of the same workweek of a group of 39 male construction workers. DNA methylation was measured by bisulphite-PCR-Pyrosequencing. We also measured the levels of trace elements in the whole blood by ICP-MS. Results Only theCDKN2Agene had significant differences in the average methylation level between the first and fifth day of the workweek. We also observed that the levels of Cu, Pb, Se, Mn, and Ti decreased during the fifth day of exposure, and only lead, titanium and copper showed a low significant correlation with the methylation level mean for three specific CpG sites of theCDKN2A. Conclusions In summary, the data suggest that altered levels ofCDKN2Amethylation in construction workers may be a potential biomarker of recent exposure in this environment. (AU)

• Processo FAPESP: 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/24279-1 - Avaliação da exposição a agentes carcinogênicos ambientais e ocupacionais em trabalhadores da construção civil
• Beneficiário: Henrique César Santejo Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular