Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

Autor(es)

Pela, Vinicius T. ^[1] ; Lunardelli, Joao G. Q. ^[2] ; Ventura, Talita M. O. ^[2] ; Camiloti, Gabriel D. ^[2] ; Baumann, Tommy ^[3] ; Carvalho, Thiago S. ^[3] ; Lussi, Adrian ^[3] ; Buzalaf, Marilia A. R. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos - Brazil

^[2] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[3] Univ Bern, Dept Restorat Prevent & Pediat Dent, Sch Dent Med, Bern - Switzerland

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Oral Sciences; v. 128, n. 6 NOV 2020.
• Citações Web of Science: 0

Resumo

This study compared the protein profile of the acquired enamel pellicle (AEP) formed under three conditions: in vitro, in situ, and in vivo. Nine volunteers participated in all procedures. In the in vitro condition, the volunteers donated saliva, in which specimens were incubated to form the AEP. In the in situ condition, the volunteers used an oral device containing specimens where the AEP was formed. In the in vivo condition, the AEP was collected from the volunteers own teeth. All AEPs were formed for 120 min, collected and processed by mass spectrometry. Overall, a total of 321 proteins were identified, among which 37 proteins are commonly considered typical in the AEP. For each of the in vitro, in situ, and in vivo conditions, respectively, 66, 174, and 170 proteins were identified. For the in vitro condition, 17 pellicle-typical proteins were not identified. Furthermore, several proteins with important functions within the AEP presented differences in expression in the three conditions. The qualitative profile of the proteins, especially the typical ones, is different in the in vitro condition. In addition, there are important quantitative differences that may interfere when attempting to extrapolate in vitro results to an in situ and in vivo condition. (AU)

• Processo FAPESP: 17/26376-8 - Perfil proteômico da película adquirida do esmalte formado in situ ou in vivo
• Beneficiário: João Guilherme Quintal Lunardelli
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/04857-4 - Engenharia de película adquirida para o controle da erosão dentária: avaliação in situ do potencial protetor de uma nova cistatina derivada da cana-de-açúcar
• Beneficiário: Vinícius Taioqui Pelá
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/12041-7 - Segurança e eficácia para prevenção da erosão dentária de uma nova cistatina derivada da cana-de-açúcar (CaneCPI-5): estudos in vitro, in situ e in vivo
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/05031-2 - Análise proteômica da película adquirida do esmalte e saliva em pacientes com câncer de cabeça e pescoço submetidos à radioterapia
• Beneficiário: Talita Mendes Oliveira Ventura
• Modalidade de apoio: Bolsas no Brasil - Doutorado