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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Proteomic profiles of the acquired enamel pellicle formed in vitro, in situ, or in vivo

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Author(s):
Pela, Vinicius T. [1] ; Lunardelli, Joao G. Q. [2] ; Ventura, Talita M. O. [2] ; Camiloti, Gabriel D. [2] ; Baumann, Tommy [3] ; Carvalho, Thiago S. [3] ; Lussi, Adrian [3] ; Buzalaf, Marilia A. R. [2]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos - Brazil
[2] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
[3] Univ Bern, Dept Restorat Prevent & Pediat Dent, Sch Dent Med, Bern - Switzerland
Total Affiliations: 3
Document type: Journal article
Source: European Journal of Oral Sciences; v. 128, n. 6 NOV 2020.
Web of Science Citations: 0
Abstract

This study compared the protein profile of the acquired enamel pellicle (AEP) formed under three conditions: in vitro, in situ, and in vivo. Nine volunteers participated in all procedures. In the in vitro condition, the volunteers donated saliva, in which specimens were incubated to form the AEP. In the in situ condition, the volunteers used an oral device containing specimens where the AEP was formed. In the in vivo condition, the AEP was collected from the volunteers own teeth. All AEPs were formed for 120 min, collected and processed by mass spectrometry. Overall, a total of 321 proteins were identified, among which 37 proteins are commonly considered typical in the AEP. For each of the in vitro, in situ, and in vivo conditions, respectively, 66, 174, and 170 proteins were identified. For the in vitro condition, 17 pellicle-typical proteins were not identified. Furthermore, several proteins with important functions within the AEP presented differences in expression in the three conditions. The qualitative profile of the proteins, especially the typical ones, is different in the in vitro condition. In addition, there are important quantitative differences that may interfere when attempting to extrapolate in vitro results to an in situ and in vivo condition. (AU)

FAPESP's process: 17/26376-8 - Proteomic profile of the acquired enamel pellicle formed in situ or in vivo
Grantee:João Guilherme Quintal Lunardelli
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/04857-4 - Acquired pellicle engineering for control of dental erosion: in situ evaluation of protective potential by a new cistatin derived from sugar cane
Grantee:Vinícius Taioqui Pelá
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 18/12041-7 - Safety and efficacy for the prevention of dental erosion of a new cystatin derived from sugarcane (CaneCPI-5): in vitro, in situ and in vivo studies
Grantee:Marília Afonso Rabelo Buzalaf
Support Opportunities: Regular Research Grants
FAPESP's process: 17/05031-2 - Proteomic analysis of the acquired enamel pellicle and saliva in patients with head and neck cancer that are submitted radiotherapy
Grantee:Talita Mendes Oliveira Ventura
Support Opportunities: Scholarships in Brazil - Doctorate