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Determination of the composition of the acquired pellicle formed on enamel and dentin and its modification after chemical and mechanic stimulation of salivary flow: proteomic study

Grant number: 11/03112-9
Support type:Scholarships in Brazil - Master
Effective date (Start): September 01, 2011
Effective date (End): August 31, 2013
Field of knowledge:Health Sciences - Dentistry
Principal researcher:Marília Afonso Rabelo Buzalaf
Grantee:Flávia Zaidan Cardoso dos Santos
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated scholarship(s):12/10137-0 - Characterization of the acquired pellicle formed in situ over human enamel and dentin before and after mechanical and chemical salivary flow stimulation: proteomic study, BE.EP.MS


The aim of this study is to compare the protein profile of acquired pellicles formed in vivo on human enamel under resting conditions (group 1), as well as to analyze the protein profile of these pellicles formed after mechanical (group 2) and chemical stimulation (group 3) of the salivary flow.A crossmatch study will be conducted in three consecutive days.On each day, before the experimental phase, the volunteers will receive a professional prophilactic teeth cleaning.On the first day, after 120 minutes without ingesting any type of food or beverage, and without any kind of salivary flow stimulation, the acquired pellicle will be harvested from the volunteer"s teeth with the help of an electrode wick filter soaked in 3% citric acid. On the second day, the volunteers will be instructed to chew on a parafin wax block during the whole 120 minutes and after that period of time the acquired pellicle will be removed the same way.On the third day, their salivary flow will be stimulated during the 120 minutes by dropping 2% citric acid every minute on the volunteer"s tongue. For each group a "pool" with the wick filters of all the volunteers will be done.After the protein extraction, they will be concentrated and put under liquid cromatography tandem mass spectrometer (LC-ESI-MS/MS). The data will be processed on the SQUEST software (Bioworks Browser 3.2, Thermo-Finnigan, São Jose, CA, USA). The search will be made using the human protein database SWISS-PROT and TrEMBL. (AU)

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