Exercise intolerance establishment in pulmonary hypertension: Preventive effect of aerobic exercise training

Autor(es)

Vieira, J. S. ^[1] ; Cunha, T. F. ^[1] ; Paixao, N. A. ^[1] ; Dourado, P. M. ^[2] ; Carrascoza, L. S. ^[1] ; Bacurau, A. V. N. ^[1] ; Brum, P. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Phys Educ & Sport, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Med Sch, Heart Inst, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 261, NOV 15 2020.
• Citações Web of Science: 0

Resumo

Aims: 1) Characterize the progression of exercise intolerance in monocrotaline-induced pulmonary hypertension (PH) in mice and 2) evaluate the therapeutic effect of aerobic exercise training (AET) on counteracting skeletal and cardiac dysfunction in PH. Main methods: Wild type C57BL6/J mice were studied in two different time points: 2 months and 4 months. Exercise tolerance was evaluated by graded treadmill exercise test. The AET was performed in the last month of treatment of 4 months' time point. Cardiac function was evaluated by echocardiography. Skeletal muscle cross-sectional area was assessed by immunofluorescence. The diameter of cardiomyocytes and pulmonary edema were quantified by staining with hematoxylin-eosin. The variables were compared among the groups by two-way ANOVA or non-paired Student's t-test. Significance level was set at p < 0.05. Key findings: After 2 months of MCT treatment, mice presented pulmonary edema, right cardiac dysfunction and left ventricle hypertrophy. After 4 months of MCT treatment, mice showed pulmonary edema, right and left cardiac dysfunction and remodeling associated with exercise intolerance and skeletal muscle atrophy. AET was able to reverse cardiac left ventricle dysfunction and remodeling, prevent exercise intolerance and skeletal muscle dysfunction. Thus, our data provide evidence of skeletal muscle abnormalities on advanced PH. AET was efficient in inducing an anti-cardiac remodeling effect besides preventing exercise intolerance. Significance: Our study provides a robust model of PH in mice, as well as highlights the importance of AET as a preventive strategy for exercise intolerance and, skeletal and cardiac muscle abnormalities in PH. (AU)

• Processo FAPESP: 15/22814-5 - Câncer e coração: novos paradigmas de diagnóstico e tratamento
• Beneficiário: Carlos Eduardo Negrão
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/24284-6 - Papel do treinamento físico aeróbico na plasticidade muscular em modelo experimental de insuficiência cardíaca por hipertensão pulmonar
• Beneficiário: Janaina da Silva Vieira
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/25957-9 - Influência da aptidão aeróbica na homeostase redox: identificação e implicação biológica da oxidação de resíduos cisteína em proteínas do músculo cardíaco e esquelético
• Beneficiário: Rodrigo Wagner Alves de Souza
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado