RNA-sequencing of the Nyssomyia neivai sialome: a sand fly-vector from a Brazilian endemic area for tegumentary leishmaniasis and pemphigus foliaceus

Autor(es)

Vernal, Sebastian ^{[1, 2]} ; Oliveira, Fabiano ^[3] ; Oliveira, Wanderson H. C. ^[4] ; Goulart, Thais M. ^[4] ; Oristian, James ^[3] ; Calvo, Eric ^[3] ; Pinto, Mara C. ^[4] ; Roselino, Ana Maria ^[2] ; Ribeiro, Jose M. C. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Infect Dis, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Clin Med, Div Dermatol, Ribeirao Preto, SP - Brazil

^[3] NIAID, Lab Malaria & Vector Res, NIH, Rockville, MD 20852 - USA

^[4] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 OCT 19 2020.
• Citações Web of Science: 1

Resumo

Leishmaniasis encompasses a spectrum of diseases caused by a protozoan belonging to the genus Leishmania. The parasite is transmitted by the bite of sand flies, which inoculate the promastigote forms into the host's skin while acquiring a blood meal. Nyssomyia neivai is one of the main vectors of tegumentary leishmaniasis (TL) in Brazil. Southeastern Brazil is an endemic region for TL but also overlaps with an endemic focus for pemphigus foliaceus (PF), also known as Fogo Selvagem. Salivary proteins of sand flies, specifically maxadilan and LJM11, have been related to pemphigus etiopathogenesis in the New World, being proposed as an environmental trigger for autoimmunity. We present a comprehensive description of the salivary transcriptome of the N. neivai, using deep sequencing achieved by the Illumina protocol. In addition, we highlight the abundances of several N. neivai salivary proteins and use phylogenetic analysis to compare with Old- and New-World sand fly salivary proteins. The collection of protein sequences associated with the salivary glands of N. neivai can be useful for monitoring vector control strategies as biomarkers of N. neivai, as well as driving vector-vaccine design for leishmaniasis. Additionally, this catalog will serve as reference to screen for possible antigenic peptide candidates triggering anti-Desmoglein-1 autoantibodies. (AU)

• Processo FAPESP: 10/51729-2 - Relação dos fatores imunogenéticos e ambientais na etiopatogênese dos pênfigos em amostragem brasileira
• Beneficiário: Ana Maria Ferreira Roselino
• Modalidade de apoio: Auxílio à Pesquisa - Temático