Immunohistochemical detection of Lp25 and LipL32 proteins in skeletal and cardiac muscles of fatal human leptospirosis

Autor(es)

Iglezias, Silvia D'Andretta ^[1] ; Estima Abreu, Patricia Antonia ^[2] ; Kanamura, Cristina ^[1] ; Magaldi, Antonio Jose ^[3] ; Seguro, Antonio Carlos ^[3] ; De Brito, Thales ^[4]

Afiliação do(s) autor(es)

^[1] Adolfo Lutz Inst, Lab Patol, Sao Paulo, SP - Brazil

^[2] Inst Butantan, Lab Bacteriol, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Hosp Clin, Dept Nefrol, Lab Invest Med LIM 12, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 62, 2020.
• Citações Web of Science: 0

Resumo

ABSTRACT Leptospirosis is an acute infection caused by pathogenic species of the genus Leptospira, which affects humans and animals in all world. In severe forms of the disease, kidneys, liver and lungs are the main affected organs, resulting in acute kidney injury, jaundice and pulmonary hemorrhage. Previous post-mortem studies have shown that lesions are not limited to these organs. Cardiac and striated muscle injuries have already been reported, but the pathophysiology of cardiac and skeletal lesions in leptospirosis is not fully understood. It has been suggested that the tissue damage observed in leptospirosis could be directly mediated by leptospires or by their toxic cellular components. LipL32 and Lp25 are leptospira membrane proteins with unknown functions, that are present only in pathogenic strains of Leptospira spp. Both proteins induce skeletal muscle lesions similar to those observed when normal guinea pigs are inoculated with leptospires. Through immunohistochemistry, this study showed the presence of LipL32 and Lp25 proteins on muscle cell membranes and in the underlying cytoplasm of skeletal muscles, as well as focal lesions in cardiac tissues of fatal cases of leptospirosis. Altogether, these results reinforce that both proteins can be important factors in the pathogenesis of leptospirosis. (AU)

• Processo FAPESP: 19/00546-0 - Estudo do potencial vacinal da proteína LigB de Leptospira spp.
• Beneficiário: Patricia Antonia Estima Abreu de Aniz
• Modalidade de apoio: Auxílio à Pesquisa - Regular