Detection of Streptococcus mutans in symptomatic and asymptomatic infected root canals

Autor(es)

Lima, Augusto Rodrigues ^{[1, 2]} ; Herrera, Daniel Rodrigo ^{[1, 3]} ; Francisco, Priscila Amanda ^[1] ; Pereira, Andrea Cardoso ^{[1, 2]} ; Lemos, Jose ^[2] ; Abranches, Jacqueline ^[2] ; Gomes, Brenda P. F. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Dept Restorat Dent, Endodont Div, Piracicaba Dent Sch, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Florida, Dept Oral Biol, Coll Dent, 1395 Ctr Dr, Box 100424, Gainesville, FL 32610 - USA

^[3] Fluminense Fed Univ UFF, Dept Endodont, Niteroi, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 6 NOV 2020.
• Citações Web of Science: 0

Resumo

Objectives To investigate the presence of Streptococcus mutans in root canals of symptomatic necrotic teeth (SNT) and their associated acute apical abscesses (AAA) and in the root canals of asymptomatic necrotic teeth (ANT). It also aimed to investigate the presence of the cnm and cbm genes in specimens that harbored S. mutans. Materials and methods DNA was extracted from samples collected from 10 patients presenting pulpal necrosis associated with radiographic evidence of apical periodontitis (ANT) and from 10 patients in need of endodontic therapy due to the presence of pulpal necrosis (SNT) and AAA. The control group consisted of 10 patients with teeth with normal vital pulp and requiring endodontic treatment for prosthetic reasons. The presence of S. mutans was detected by quantitative real-time-PCR (qPCR) using species-specific primers. Samples harboring S. mutans were further evaluated for the presence of CBP genes by qPCR as well. Results All studied sites showed a high prevalence of S. mutans, except the control group. Specifically, 60% of ANT and 70% of AAA/SNT paired samples were positive for S. mutans. The cnm gene was detected positive for S. mutans only in ANT samples (66.6%). The cbm gene was not detected in any of the investigated sites. Conclusions S. mutans was found in high prevalence in both asymptomatic and symptomatic endodontic infections, including in abscesses, but it was not detected in the root canals of teeth with normal vital pulp. Interestingly, cnm(+)S. mutans was only detected in asymptomatic/chronic primary endodontic infections associated with apical lesion. Therefore, it appears that cnm, and possibly other CBPs, may play an underestimated role in chronic endodontic infections. (AU)

• Processo FAPESP: 14/27366-8 - Análise do conteúdo bacteriano e suscetibilidade de bactérias específicas frente a agentes antimicrobianos
• Beneficiário: Augusto Rodrigues Lima
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/23950-2 - Monitoramento do conteúdo infeccioso, endotóxico e da presença de genes de resistência a antibióticos de infecções primárias sintomáticas e assintomáticas durante a terapia endodôntica
• Beneficiário: Augusto Rodrigues Lima
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/18512-6 - Detecção de Streptococcus mutans e da glicoproteína Cnm de canais radiculares infectados associados a abscessos dentários
• Beneficiário: Augusto Rodrigues Lima
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado