Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detection of Streptococcus mutans in symptomatic and asymptomatic infected root canals

Full text
Author(s):
Lima, Augusto Rodrigues [1, 2] ; Herrera, Daniel Rodrigo [1, 3] ; Francisco, Priscila Amanda [1] ; Pereira, Andrea Cardoso [1, 2] ; Lemos, Jose [2] ; Abranches, Jacqueline [2] ; Gomes, Brenda P. F. A. [1]
Total Authors: 7
Affiliation:
[1] Univ Campinas UNICAMP, Dept Restorat Dent, Endodont Div, Piracicaba Dent Sch, Av Limeira 901, BR-13414903 Piracicaba, SP - Brazil
[2] Univ Florida, Dept Oral Biol, Coll Dent, 1395 Ctr Dr, Box 100424, Gainesville, FL 32610 - USA
[3] Fluminense Fed Univ UFF, Dept Endodont, Niteroi, RJ - Brazil
Total Affiliations: 3
Document type: Journal article
Source: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 6 NOV 2020.
Web of Science Citations: 0
Abstract

Objectives To investigate the presence of Streptococcus mutans in root canals of symptomatic necrotic teeth (SNT) and their associated acute apical abscesses (AAA) and in the root canals of asymptomatic necrotic teeth (ANT). It also aimed to investigate the presence of the cnm and cbm genes in specimens that harbored S. mutans. Materials and methods DNA was extracted from samples collected from 10 patients presenting pulpal necrosis associated with radiographic evidence of apical periodontitis (ANT) and from 10 patients in need of endodontic therapy due to the presence of pulpal necrosis (SNT) and AAA. The control group consisted of 10 patients with teeth with normal vital pulp and requiring endodontic treatment for prosthetic reasons. The presence of S. mutans was detected by quantitative real-time-PCR (qPCR) using species-specific primers. Samples harboring S. mutans were further evaluated for the presence of CBP genes by qPCR as well. Results All studied sites showed a high prevalence of S. mutans, except the control group. Specifically, 60% of ANT and 70% of AAA/SNT paired samples were positive for S. mutans. The cnm gene was detected positive for S. mutans only in ANT samples (66.6%). The cbm gene was not detected in any of the investigated sites. Conclusions S. mutans was found in high prevalence in both asymptomatic and symptomatic endodontic infections, including in abscesses, but it was not detected in the root canals of teeth with normal vital pulp. Interestingly, cnm(+)S. mutans was only detected in asymptomatic/chronic primary endodontic infections associated with apical lesion. Therefore, it appears that cnm, and possibly other CBPs, may play an underestimated role in chronic endodontic infections. (AU)

FAPESP's process: 14/27366-8 - Analysis of the bacterial and the antimicrobial susceptibility of selected bacteria against antimicrobial agentes
Grantee:Augusto Rodrigues Lima
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 16/23950-2 - Monitoring of infectious contents, endotoxic and gene presence of resistance to antibiotics of symptomatic and asymptomatic primary infections during endodontic therapy
Grantee:Augusto Rodrigues Lima
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/18512-6 - Detection of Streptococcus mutans and glycoprotein Cnm from infected root canals and their associated dental abscesses
Grantee:Augusto Rodrigues Lima
Support Opportunities: Scholarships abroad - Research Internship - Master's degree