Cancer-associated fibroblast regulation by microRNAs promotes invasion of oral squamous cell carcinoma

Autor(es):Mostrar menos -

Matos, Leandro Luongo ^[1] ; Menderico Junior, Gilberto Mendes ^[2] ; Theodoro, Therese Rachell ^[3] ; Pasini, Fatima Solange ^[4] ; Ishikawa, Marina de Menezes ^[5] ; Bomtempo Ribeiro, Andromeda Aryane ^[6] ; de Mello, Evandro Sobroza ^[7] ; da Silva Pinhal, Maria Aparecida ^[3] ; Moyses, Raquel Ajub ^[8] ; Vamondes Kulcsar, Marco Aurelio ^[9] ; Dedivitis, Rogerio Aparecido ^[10] ; Cernea, Claudio Roberto ^[10] ; Kowalski, Luiz Paulo ^{[10, 11]}

Afiliação do(s) autor(es):Mostrar menos -

^[1] Univ Sao Paulo, Inst Canc Estado Sao Paulo ICESP, Head & Neck Surg Dept, Lab Invest Med 28 LIM28, Med Sch, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Head & Neck Surg Dept, PhD Program, Med Sch, Sao Paulo - Brazil

^[3] Fac Med ABC, Biochem Dept, Santo Andre, SP - Brazil

^[4] Univ Sao Paulo HCFMUSP, Ctr Invest Translac Oncol CTO, Inst Canc Estado Sao Paulo ICESP, Hosp Clin, Fac Med, Sao Paulo - Brazil

^[5] Fac Israelita Ciencias Saude Albert Einstein, Nursery Sch, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Med Sch, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Inst Canc Estado Sao Paulo ICESP, Pathol Dept, Lab Invest Med 14 LIM14, Med Sch, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Head & Neck Surg Dept, Lab Invest Med 28 LIM28, Med Sch, Sao Paulo - Brazil

^[9] Univ Sao Paulo, Inst Canc Estado Sao Paulo ICESP, Head & Neck Surg Dept, Med Sch, Sao Paulo - Brazil

^[10] Univ Sao Paulo, Head & Neck Surg Dept, Med Sch, Sao Paulo - Brazil

^[11] Univ Sao Paulo, Surg Dept, Head & Neck Surg Discipline, Med Sch, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Oral Oncology; v. 110, NOV 2020.
• Citações Web of Science: 0

Resumo

The objective of the present study was to evaluate the role of microRNA-mediated remodeling of the extracellular matrix in the process of tumor invasion of oral squamous cell carcinoma and to evaluate its relationship with the prognosis of these patients. This was a retrospective study on material from the paraffin blocks of patients operated on for oral squamous cell carcinoma, in addition to a group of healthy oral mucosa samples of paired patients. miR-1-3p, miR-133-3p, and miR-21-5p were differentially expressed between the superficial and deep tumor groups. miR-21-5p was the one with the greatest accuracy in the differentiation between superficial and deep tumors. By immunohistochemistry, the group of deep tumors showed greater immunoreactivity to matrix metalloproteinases 2 and 9 and laminin a in tumor-associated fibroblasts, with consequent degradation of the basal membrane, measured by greater loss of continuity of type IV collagen. This process was also associated with lower and higher expression of miR-1-3p and miR-21-5p, respectively. There was also a trend toward better overall and disease-free survival rates in patients with higher miR-133a-3p. The present study showed the interaction between microRNAs and extracellular matrix remodeling in oral squamous cell carcinoma. (AU)

• Processo FAPESP: 16/01740-6 - PERFIL DE PROTEOGLICANOS E EXPRESSÃO DE MICRO-RNAs ASSOCIADOS A INVASÃO TUMORAL E PROGNÓSTICO EM CARCINOMA ESPINOCELULAR DE CAVIDADE ORAL
• Beneficiário: Leandro Luongo de Matos
• Modalidade de apoio: Auxílio à Pesquisa - Regular