Correlation between clinical outcome and tissue inflammatory response in kidney transplant recipients with cryptococcosis

Autor(es)

Nishikaku, Angela S. ^[1] ; Solda, V, Marcel ; Ricci, Giannina ^[2] ; Ponzio, Vinicius ^{[2, 3]} ; Pagliari, Carla ^[4] ; Medina-Pestana, Jose O. ^[3] ; de Franco, Marcello F. ^[5] ; Colombo, Arnaldo Lopes ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Med, Lab Especial Micol, Disciplina Infectol, Sao Paulo, SP - Brazil

^[2] Solda, Marcel, V, Univ Fed Sao Paulo, Dept Med, Lab Especial Micol, Disciplina Infectol, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Hosp Rim, Fundacao Oswaldo Ramos, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Fac Med, Dept Patol, Sao Paulo, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Patol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS AND DISEASE; v. 78, n. 7 OCT 2020.
• Citações Web of Science: 0

Resumo

Cryptococcosis is the second most common invasive fungal infection reported in renal transplant recipients. Tissue granulomatous inflammation is necessary to contain Cryptococcus infection. This study aims to analyze the granuloma patterns and in situ expression of regulatory T (Treg) immune response in tissue samples from 12 renal transplant recipients with cryptococcosis. Fungal isolates were molecularly identified as Cryptococcus neoformans species complex. A detailed characterization of granulomas in tissue samples from 12 kidney transplant recipients with cryptococcosis was described by checking six lung and six skin biopsies by conventional histology and for immunohistochemical detection of CD4 and Treg markers: forkhead box P3 (FoxP3), interleukin (IL)-10 and transforming-growth factor (TGF)-beta. Granulomas were classified as compact, loose or mixed. Patients with mixed (n = 4) and compact (n = 3) granulomatous inflammation patterns were associated with a better prognosis and presented a higher number of CD4(+)FoxP3(+)T cells compared to the group of patients with loose granulomas. In counterpart, three out of five patients with loose granulomas died with cryptococcosis. We suggest that Treg may have a protective role in the tissue response to Cryptococcus infection given its association with compact and mixed granulomas in patients with better clinical outcomes. (AU)

• Processo FAPESP: 13/01280-7 - Caracterização da resposta imune tecidual em pacientes pós-transplante renal com infecção fúngica causada pelo complexo de espécies Cryptococcus neoformans / Cryptococcus gattii
• Beneficiário: Marcello Fabiano de Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular