Angiotensin (1-7)-attenuated vasoconstriction is associated with the Interleukin-10 signaling pathway

Autor(es)

Freitas, Raiany A. ^[1] ; Junior, Rinaldo R. P. ^[1] ; Justina, Vanessa D. ^[1] ; Bressan, Alecsander F. M. ^[2] ; Bomfim, Gisele F. ^[3] ; Carneiro, Fernando S. ^[2] ; Giachini, Fernanda R. ^[1] ; Lima, Victor V. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Mato Grosso, Inst Biol & Hlth Sci, Ave Valdon Varjao, BR-78600000 Barra Do Garcas, MT - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto, SP - Brazil

^[3] Univ Fed Mato Grosso, Inst Hlth Sci, Sinop, MT - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 262, DEC 1 2020.
• Citações Web of Science: 0

Resumo

Aims: Angiotensin-1-7 {[}Ang-(1-7)] is an essential peptide of the renin-angiotensin system that promotes benefits modulating effects in different tissues. Similarly, interleukin-10 (IL-10) exhibits an immunomodulatory action on the vasculature. This study aimed to evaluate whether Ang-(1-7) levels attenuates vascular contractile response, mediated by IL-10-pathway (JAK1/STAT3/IL-10). Main methods: Aortas from male mice C57BL/6J and knockout for IL-10 (IL-10(-/-)) were incubated with Ang-(1-7) {[}10 mu M] or vehicle, during 5 min, 1 h, 6 h, 12 h, and 24 h. Concentration-response curves to phenylephrine, western blotting, and flow cytometry analysis was performed to evaluate the contractile response, protein expression, and IL-10 levels, respectively. Key findings: Incubation with Ang-(1-7) produced a time-dependent increase in Janus kinases 1 (JAK1) expression, as well as increased expression and activity of the signal transducer and activator of transcription 3 (STAT3) protein. However, this effect was not observed in knockout animals for IL-10. After 12 h of Ang-(1-7) treatment, arteries from control mice displayed decreased vascular reactivity to phenylephrine, but this effect was not observed in the absence of endogenous IL-10. Additionally, incubation with Ang-(1-7) augments IL-10 levels after 6 h, 12 h, and 24 h of incubation. Significance: These results demonstrated the role of Ang-(1-7) in the IL-10 signaling pathway and its effects in the vascular contractility response. Thus, these findings suggest a new synergic action where Ang-(1-7) and IL-10 converge into a protective mechanism against vascular dysfunction. (AU)

• Processo FAPESP: 16/11988-5 - Contribuição do NLRP3 para o efeito pró-inflamatório da ET-1 em células de músculo liso do corpo cavernoso: relevância na função erétil.
• Beneficiário: Fernando Silva Carneiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular