Potential of mucoadhesive nanocapsules in drug release and toxicology in zebrafish

Autor(es):Mostrar menos -

Charlie-Silva, Ives ^[1] ; Feitosa, Natalia Martins ^[2] ; Mendonca Gomes, Juliana Moreira ^[3] ; de Melo Hoyos, Daniela Chemim ^[4] ; Mattioli, Cristiano Campos ^[5] ; Eto, Silas Fernandes ^[6] ; Fernandes, Dayanne Carla ^[7] ; de Andrade Belo, Marco Antonio ^{[8, 5]} ; Silva, Juliana de Oliveira ^[9] ; Branco de Barros, Andre Luis ^[9] ; Correa Junior, Jose Dias ^[3] ; de Menezes, Gustavo Batista ^[3] ; Silva Fukushima, Hirla Costa ^[10] ; Dias Castro, Tassia Flavia ^[10] ; Borra, Ricardo Carneiro ^[10] ; Pierezan, Felipe ^[11] ; Silva de Melo, Nathalie Ferreira ^[12] ; Fraceto, Leonardo Fernandes ^[13]

Afiliação do(s) autor(es):Mostrar menos -

^[1] Univ Sao Paulo ICB USP, Dept Pharmacol, Sao Paulo, SP - Brazil

^[2] Univ Fed Rio de Janeiro UFRJ, Inst Biodiversidade & Sustentabilidade NUPEM, Lab Integrad Biociencias Translacionais LIBT, Macae, RJ - Brazil

^[3] ICB UFMG, Dept Morphol, Belo Horizonte, MG - Brazil

^[4] Univ Fed Minas Gerais, Aquiculture Lab Laqua, Belo Horizonte, MG - Brazil

^[5] Sao Paulo State Univ, Dept Prevent Vet Med, Jaboticabal, SP - Brazil

^[6] Fed Univ Roraima UFRR, Dept Postgrad Hlth SciencesPROCISA, Boa Vista, Parana - Brazil

^[7] Sao Paulo State Univ, Inst Chem, UNESP, Araraquara, SP - Brazil

^[8] Brasil Univ, Lab Anim Pharmacol & Toxicol, Descalvado, SP - Brazil

^[9] Univ Fed Minas Gerais, Dept Clin & Toxicol Anal, Fac Pharm, Belo Horizonte, MG - Brazil

^[10] Univ Fed Sao Carlos, Lab Appl Immunol, Sao Carlos, SP - Brazil

^[11] Univ Fed Minas Gerais, Dept Clin & Vet Surg, Sch Vet Med, Belo Horizonte, MG - Brazil

^[12] Fac Med Sao Leopoldo Mandic, Araras, SP - Brazil

^[13] Sao Paulo State Univ, Inst Sci & Technol Sorocaba, Sorocaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 15, n. 9 SEP 24 2020.
• Citações Web of Science: 1

Resumo

Mucoadhesive polymeric nanocapsules have attracted interest of researchers from different fields from natural sciences because of their ability to interact with the mucosa and increase drug permeation. Anesthesia by immersion causes absorption through the skin and gills of fish, so it is important to evaluate the exposure of these organs to drug nanosystems. Benzocaine (BENZ) is one of the most popular anesthetic agents used in fish anesthesia, but it has drawbacks because of its low bioavailability, resulting in weak absorption after immersion. Here we describe method developed for preparing and characterizing chitosan-coated PLGA mucoadhesive nanoparticles containing BENZ (NPMAs) for zebrafish immersion anesthesia. We determined the lowest effective concentration, characterized the interaction of the mucoadhesive system with fish, measured the anesthetic efficacy, and evaluated possible toxic effects in embryos and adults exposed to the nanoformulations. This study opens perspectives for using nanoformulations prepared with BENZ in aquaculture, allowing reduction of dosage as well as promoting more effective anesthesia and improved interaction with the mucoadhesive system of fish. (AU)

• Processo FAPESP: 19/19939-1 - Efeitos de fármacos marinhos em células ZFL
• Beneficiário: Ives Charlie da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/25971-9 - Adaptação e desenvolvimento de um modelo experimental para estudos da inflamação em Danio rerio (zebrafish)
• Beneficiário: Melque Ferrari de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/07098-0 - Efeito de fármacos marinhos em Peixe Zebra e na linhagem ZFL - emphasis
• Beneficiário: Leticia Veras Costa Lotufo
• Modalidade de apoio: Auxílio à Pesquisa - Regular