The Protein Corona Conundrum: Exploring the Advantages and Drawbacks of its Presence around Amphiphilic Nanoparticles

Autor(es)

de Castro, Carlos E. ^[1] ; Panico, Karine ^[1] ; Stangherlin, Lucas M. ^[1] ; Ribeiro, Caroline A. S. ^[1] ; da Silva, Maria C. C. ^[1] ; Carneiro-Ramos, Marcela S. ^[1] ; Dal-Bo, Alexandre G. ^[2] ; Giacomelli, Fernando C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil

^[2] Univ Extremo Sul Catarinense, BR-88806000 Criciuma, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOCONJUGATE CHEMISTRY; v. 31, n. 11, p. 2638-2647, NOV 18 2020.
• Citações Web of Science: 0

Resumo

The success of targeted drug delivery systems still requires a detailed understanding about the biological consequences of self-developed biomolecular coronas around them, since this is the surface that interacts with living cells. Herein, we report the behavior of carbohydrate-decorated amphiphilic nanoparticles in a plasma environment with regard to the formation and biological consequences of the protein corona. Naked amphiphilic nanoparticles were produced through the self-assembly of azido-PEO900-docosanoate molecules, and the coupling of N-acetylglucosamine via click chemistry enabled the fabrication of the corresponding bioactive glyco-nanostructures. Light scattering measurements, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, liquid chromatography-mass spectrometry, and the Pierce BCA protein assay all confirmed the presence of protein coronas around the self-assembled nanoparticles, regardless of the presence of the sugar residues, although it reduces the amount of adsorbed proteins. The protein coronas were formed mainly by human serum albumin, complement proteins, apolipoproteins, immunoglobulins, and proteins involved in the coagulation cascade (fibrinogen and prothrombin). While the presence of these protein coronas significantly reduced cellular uptake of the amphiphilic assemblies, they also notably reduced the cytotoxic and hemolytic effects that result from the contact of the nanoparticles with living cells. Accordingly, we highlight that protein coronas should not always be treated as artifacts that have to be avoided because they can also provide beneficial effects. (AU)

• Processo FAPESP: 19/06634-8 - Vesículas poliméricas sensíveis a estímulos ambientais como plataformas alternativas em terapias contra o câncer
• Beneficiário: Fernando Carlos Giacomelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/24686-4 - Investigação de efeitos de superfície e presença de coroa protéica no processo de internalização celular de sistemas poliméricos nanoestruturados
• Beneficiário: Carlos Eduardo de Castro
• Modalidade de apoio: Bolsas no Brasil - Doutorado