The co-infection with Ehrlichia minasensis, Anaplasma marginale and Anaplasma platys is not associated with anemia in beef cattle in the Brazilian Pantanal

Autor(es)

Andre, Marcos Rogerio ^[1] ; Calchi, Ana Claudia ^[1] ; Herrera, Heitor Miraglia ^[2] ; de Souza Zanatto, Diego Carlos ^[1] ; Le Senechal Horta, Bruna de Carvalho ^[1] ; Tasso, Julia Banhareli ^[1] ; de Souza Ramos, Inalda Angelica ^[1] ; Califre de Mello, Victoria Valente ^[1] ; Machado, Rosangela Zacarias ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Ciencias Agr & Vet, Dept Patol Vet, Jaboticabal, SP - Brazil

^[2] Univ Catolica Dom Bosco, Campo Grande, MS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS; v. 21, JUL 2020.
• Citações Web of Science: 1

Resumo

The Anaplasmataceae family is composed of obligatory intracellular Gram-negative bacteria transmitted by arthropod vectors. In Brazil, with the exception of Anaplasma marginale, little is known about the occurrence of other Anaplasma and Ehrlichia species infecting cattle. The present study aimed at investigating the occurrence of Anaplasma spp. and Ehrlichia spp. in beef cattle (Bos indicus) sampled in the Brazilian Pantanal, an area prone to periodic flooding and endemic for bovine trypanosomiasis. Blood samples from 400 cattle were collected and screened by PCR assays based on rrs and dsb genes from Anaplasma spp. and Ehrlichia spp., respectively. Positive samples for Anaplasma spp. were subjected to qPCR assays based on the msp-2 gene and nPCR based on the groEL gene. As a result, 4.75% (19/400) and 48.12% (167/347) were positive for Anaplasma platys and Ehrlichia minasensis, respectively. Besides, positivity of 56.75% (227/400) for A. marginale and seropositivity of 90.75% (363/400) for Trypanosoma vivax were found. A high rate of co-infection was observed (67.25%), from which the co-infection by A. marginale and E. minasensis was more frequently found in calves than cows. Interestingly, none of the animals presenting co-infection showed anemia or other clinical signs. The present study showed, for the first time, the occurrence of A. platys and E. minasensis in beef cattle in the southern Pantanal, as well as a high rate of co-infection by A. marginale, E. minasensis and T. vivax in the sampled animals. (AU)

• Processo FAPESP: 18/13838-6 - Detecção e caracterização molecular de agentes Anaplasmataceae em Xenarthra no Brasil
• Beneficiário: Ana Cláudia Calchi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/23831-6 - Ocorrência de Coxiella burnetii em cervos-do-Pantanal (Blastocerus dichotomus) de vida livre no Brasil
• Beneficiário: Júlia Banhareli Tasso
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/23327-6 - Detecção molecular e sorológica de Anaplasma marginale em bovinos de corte amostrados no Pantanal sul matogrossense
• Beneficiário: Bruna de Carvalho Le Sénéchal Horta
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/02753-0 - ISOLAMENTO E GENOTIPAGEM DE Bartonella spp. EM MAMIFEROS RESERVATÓRIOS DOMÉSTICOS E SELVAGENS NO BRASIL
• Beneficiário: Marcos Rogério André
• Modalidade de apoio: Auxílio à Pesquisa - Regular