The co-infection with Ehrlichia minasensis, Anaplasma marginale and Anaplasma platys is not associated with anemia in beef cattle in the Brazilian Pantanal

Author(s)

Andre, Marcos Rogerio ^[1] ; Calchi, Ana Claudia ^[1] ; Herrera, Heitor Miraglia ^[2] ; de Souza Zanatto, Diego Carlos ^[1] ; Le Senechal Horta, Bruna de Carvalho ^[1] ; Tasso, Julia Banhareli ^[1] ; de Souza Ramos, Inalda Angelica ^[1] ; Califre de Mello, Victoria Valente ^[1] ; Machado, Rosangela Zacarias ^[1]

Affiliation

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Ciencias Agr & Vet, Dept Patol Vet, Jaboticabal, SP - Brazil

^[2] Univ Catolica Dom Bosco, Campo Grande, MS - Brazil

• Document type: Journal article
• Source: VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS; v. 21, JUL 2020.
• Web of Science Citations: 1

Abstract

The Anaplasmataceae family is composed of obligatory intracellular Gram-negative bacteria transmitted by arthropod vectors. In Brazil, with the exception of Anaplasma marginale, little is known about the occurrence of other Anaplasma and Ehrlichia species infecting cattle. The present study aimed at investigating the occurrence of Anaplasma spp. and Ehrlichia spp. in beef cattle (Bos indicus) sampled in the Brazilian Pantanal, an area prone to periodic flooding and endemic for bovine trypanosomiasis. Blood samples from 400 cattle were collected and screened by PCR assays based on rrs and dsb genes from Anaplasma spp. and Ehrlichia spp., respectively. Positive samples for Anaplasma spp. were subjected to qPCR assays based on the msp-2 gene and nPCR based on the groEL gene. As a result, 4.75% (19/400) and 48.12% (167/347) were positive for Anaplasma platys and Ehrlichia minasensis, respectively. Besides, positivity of 56.75% (227/400) for A. marginale and seropositivity of 90.75% (363/400) for Trypanosoma vivax were found. A high rate of co-infection was observed (67.25%), from which the co-infection by A. marginale and E. minasensis was more frequently found in calves than cows. Interestingly, none of the animals presenting co-infection showed anemia or other clinical signs. The present study showed, for the first time, the occurrence of A. platys and E. minasensis in beef cattle in the southern Pantanal, as well as a high rate of co-infection by A. marginale, E. minasensis and T. vivax in the sampled animals. (AU)

• FAPESP's process: 18/13838-6 - Molecular detection and characterization of Anaplasmataceae agents in Xenarthra in Brazil
• Grantee: Ana Cláudia Calchi
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/23831-6 - Occurrence of Coxiella burnetii in free living marsh deer (Blastocerus dichotomus) in Brazil
• Grantee: Júlia Banhareli Tasso
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/23327-6 - Molecular and sorological detection of Anaplasma marginale in beef cattle from Pantanal sul matogrossense
• Grantee: Bruna de Carvalho Le Sénéchal Horta
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/02753-0 - ISOLATION AND GENOTYPING OF Bartonella spp. IN DOMESTIC AND WILD RESERVOIR MAMMALS IN BRAZIL
• Grantee: Marcos Rogério André
• Support Opportunities: Regular Research Grants