Molecular detection and characterization of Anaplasmataceae agents in Xenarthra in Brazil

• Grant number: 18/13838-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2018
• End date: July 31, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Marcos Rogério André
• Grantee: Ana Cláudia Calchi
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The Anaplasmataceae family comprises obligate intracellular Gram-negative bacteria, parasites of blood cells and most of which are transmitted by ticks. Although animals of the Xenarthra Order are considered as reservoirs of a wide range of zoonotic agents, few studies have investigated the possible role of these animals as hosts for the highlighted pathogens. The present study aims to verify, using molecular techniques, the occurrence and genetic diversity of Anaplasmataceae agents in Xenarthra in Brazil. To this purpose, 355 blood / tissue samples from wild sloths, anteaters and armadillos from the states of Mato Grosso do Sul, Pará, Rondônia and São Paulo will be analyzed. The biological samples will be submitted to real-time and conventional PCR assays based on the 16S rRNA, groESL, dsb, gltA, omp-1, mpa-1, sodB, msp-2 and vlpt genes. The phylogenetic positioning of the sequences obtained will be performed through the construction of dendograms. Finally, the sequence alignment will be used to calculate the nucleotide diversity and the level of polymorphisms for the analysis of the genetic diversity of the Anaplasmataceae agents. The genealogy of the nucleotide sequences will be performed using the Splitstree program. This study will contribute to the study of the diversity of tick-borne agents among Brazilian wildlife.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CALCHI, ANA CLAUDIA; VULTAO, JULIANA GABOARDI; ALVES, MARIO HENRIQUE; YOGUI, DEBORA REGINA; JEAN DESBIEZ, ARNAUD LEONARD; DE SANTI, MARIELE; SANTANA, MATHEUS DE SOUZA; VIEIRA DA SILVA, THIAGO MERIGHI; WERTHER, KARIN; GERALDES TEIXEIRA, MARTA MARIA; et al. Ehrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel `Candidatus Anaplasma spp.'. SCIENTIFIC REPORTS, v. 10, n. 1, JUL 28 2020. (18/13838-6, 18/02753-0)

ANDRE, MARCOS ROGERIO; CALCHI, ANA CLAUDIA; HERRERA, HEITOR MIRAGLIA; DE SOUZA ZANATTO, DIEGO CARLOS; LE SENECHAL HORTA, BRUNA DE CARVALHO; TASSO, JULIA BANHARELI; DE SOUZA RAMOS, INALDA ANGELICA; CALIFRE DE MELLO, VICTORIA VALENTE; MACHADO, ROSANGELA ZACARIAS. The co-infection with Ehrlichia minasensis, Anaplasma marginale and Anaplasma platys is not associated with anemia in beef cattle in the Brazilian Pantanal. VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS, v. 21, JUL 2020. (18/13838-6, 17/23831-6, 17/23327-6, 18/02753-0)

CALCHI, ANA CLAUDIA; VULTAO, JULIANA GABOARDI; ALVES, MARIO HENRIQUE; YOGUI, DEBORA REGINA; DESBIEZ, ARNAUD LEONARD JEAN; AMARAL, RENAN BRESSIANINI; SANTI, MARIELE; TEIXEIRA, MARTA MARIA GERALDES; WERTHER, KARIN; MACHADO, ROSANGELA ZACARIAS; et al. Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra. TRANSBOUNDARY AND EMERGING DISEASES, v. 67, n. 5, MAR 2020. (18/02753-0, 18/13838-6)

CALCHI, ANA CLAUDIA; VULTAO, JULIANA GABOARDI; ALVES, MARIO HENRIQUE; YOGUI, DEBORA REGINA; JEAN DESBIEZ, ARNAUD LEONARD; DE SANTI, MARIELE; SANTANA, MATHEUS DE SOUZA; VIEIRA DA SILVA, THIAGO MERIGHI; WERTHER, KARIN; GERALDES TEIXEIRA, MARTA MARIA; et al. Ehrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel 'Candidatus Anaplasma spp.'. SCIENTIFIC REPORTS, v. 10, n. 1, p. 15-pg., 2020-07-28. (18/13838-6, 18/02753-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CALCHI, Ana Cláudia. Detecção e caracterização molecular de agentes Anaplasmatceae em Xenarthra no Brazil. 2020. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.