Genetic diversity of vector-borne agents in coatis (Nasua nasua) in a periurban region of Central-Western Brazil

• Grant number: 19/15150-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2019
• End date: March 31, 2023
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Marcos Rogério André
• Grantee: Lívia Perles
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated scholarship(s): 21/14244-5 - Investigation of filariids in blood, ectoparasites and skin samples from coatis (Nasua nasua) in Campo Grande, BE.EP.DR

Abstract

The coatis (Nasua nasua), that belong to the order Carnivora and family Procyonidae, are widely distributed in South America. They are animals that adapt easily to anthropized environments, making possible close contact with domestic animals and human. The families Bartonellaceae and Anaplasmataceae comprise facultative and obligatory Gram-negative, intracellular alpha-proteobacteria, respectively, that have been identified in a wide variety of mammals, including humans. Hemotrophic mycoplasmas are Gram-negative bacteria, which can range from asymptomatic infections to severe hemolytic anemia, both in animals and humans. The piroplasmids are intracellular protozoa belonging to the Apicomplexa Phylum, Order Piroplasmida and genera Theileria, Babesia, Rangelia and Cytauxzoon. Little is known about the occurrence of the abovementioned agents in quatis and their ectoparasites in Brazil. The present study aims to investigate the genetic diversity of agents transmitted by arthropods (Anaplasmataceae, Mycoplasmaceae, Bartonellaceae, Babesiidae and Theileriidae) in quatis and their ectoparasites sampled in the periurban region of Campo Grande (MS, Brazil), by means of a longitudinal study. Molecular methods will be used to evaluate the occurrence and genetic diversity of the aforementioned agents in quatis blood samples and their respective ectoparasites; additionally, isolation of Bartonella spp. from quatis blood samples using liquid and solid culture medium. Subsequently, phylogenetic inferences and evaluation of the genotype diversity of the agents under study in quatis and ectoparasite blood samples will be traced throughout a year of sampling. In this way, the results obtained will contribute to the diagnosis and genetic characterization of the aforementioned agents, besides contributing to the understanding of the possible role of quatis in the epidemiological cycles of borne vectors agents. (AU)

Scientific publications

(11)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

PERLES, L.; DE MACEDO, G. C.; BARRETO, W. T. G.; FRANCISCO, G., V; HERRERA, H. M.; BARROS-BATTESTI, D. M.; MACHADO, R. Z.; ANDRE, M. R.. Longitudinal dynamics and health impact of Hepatozoon procyonis (Apicomplexa: Hepatozoidae) on naturally infected ring-tailed coatis Nasua nasua (Carnivora: Procyonidae) from Midwestern Brazil. TICKS AND TICK-BORNE DISEASES, v. 13, n. 5, p. 11-pg., 2022-09-01. (20/12037-0, 18/02753-0, 19/15150-4)

PERLES, LIVIA; BARRETO, WANESSA TEIXEIRA GOMES; DE MACEDO, GABRIEL CARVALHO; HERRERA, HEITOR MIRAGLIA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Neorickettsia sp. in coatis (Nasua nasua) in Brazil. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 32, n. 3, p. 4-pg., 2023-01-01. (18/02753-0, 20/12037-0, 19/15150-4)

PERLES, LIVIA; BASSINI-SILVA, RICARDO; JACINAVICIUS, FERNANDO CASTRO; BARRETO, WANESSA TEIXEIRA GOMES; DE MACEDO, GABRIEL CARVALHO; MARTINS, FILIPE SANTOS; HERRERA, HEITOR MIRAGLIA; MACHADO, ROSANGELA ZACARIAS; BARROS-BATTESTI, DARCI MORAES; ANDRE, MARCOS ROGERIO. Contribution to the knowledge of Neotrichodectes (Nasuicola) pallidus (Piaget, 1880) (Phthiraptera: Trichodectidae). VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS, v. 41, p. 6-pg., 2023-06-01. (19/19853-0, 17/01416-7, 20/12037-0, 20/11755-6, 18/24667-8, 19/15150-4)

PERLES, L.; OTRANTO, D.; BARRETO, W. T. G.; DE MACEDO, G. C.; LIA, R. P.; MENDOZA-ROLDAN, J. A.; HERRERA, H. M.; DE OLIVEIRA, C. E.; MACHADO, R. Z.; ANDRE, M. R.. Mansonella sp. and associated Wolbachia endosymbionts in ring-tailed coatis (Nasua nasua) in periurban areas from Midwestern Brazil. INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE, v. 22, p. 6-pg., 2023-12-01. (20/12037-0, 18/02753-0, 21/14244-5, 19/15150-4)

PERLES, LIVIA; HERRERA, HEITOR M.; BARRETO, WANESSA T. G.; DE MACEDO, GABRIEL C.; CALCHI, ANA C.; MACHADO, ROSANGELA Z.; ANDRE, MARCOS R.. Multi-Locus Sequencing Reveals Putative Novel Anaplasmataceae Agents, 'Candidatus Ehrlichia dumleri' and Anaplasma sp., in Ring-Tailed Coatis (Carnivora: Nasua nasua) from Urban Forested Fragments at Midwestern Brazil. MICROORGANISMS, v. 10, n. 12, p. 15-pg., 2022-12-01. (20/12037-0, 18/02753-0, 19/15150-4)

PERLES, LIVIA; IKEDA, PRISCILA; FRANCISCO, GABRIELA DE VASCONCELLOS; TORRES, JAIRE MARINHO; DE OLIVEIRA, CARINA ELISEI; LOURENCO, ELIZABETE CAPTIVO; HERRERA, HEITOR MIRAGLIA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Molecular detection of Hepatozoon spp. in non-hematophagous bats in Brazil. TICKS AND TICK-BORNE DISEASES, v. 11, n. 3, MAY 2020. (18/02753-0, 19/13232-3, 17/14124-4, 19/15150-4)

PERLES, L.; MORAES, M. F.; DA SILVA, M. XAVIER; VIEIRA, R. F. C.; MACHADO, R. Z.; HOPPE, E. G. LUX; ANDRE, M. R.. Co-infection by multiple vector-borne agents in wild ring-tailed coatis (Nasua nasua) from Iguacu National Park, southern Brazil. SCIENTIFIC REPORTS, v. 13, n. 1, p. 11-pg., 2023-02-01. (16/14886-9, 16/15589-8, 19/15150-4, 18/02753-0)

PERLES, L.; BARRETO, W. T. G.; DE MACEDO, G. C.; CALCHI, A. C.; BEZERRA-SANTOS, M.; MENDOZA-ROLDAN, J. A.; OTRANTO, D.; HERRERA, H. M.; BARROS-BATTESTI, D. M.; MACHADO, R. Z.; et al. Molecular detection of Babesia spp. and Rickettsia spp. in coatis (Nasua nasua) and associated ticks from midwestern Brazil. Parasitology Research, v. 122, n. 5, p. 8-pg., 2023-03-09. (18/02753-0, 20/12037-0, 19/15150-4)

ANDRE, MARCOS ROGERIO; CALCHI, ANA CLAUDIA; PERLES, LIVIA; GONCALVES, LUIZ RICARDO; UCCELLA, LUCAS; BATISTA LEMES, JHESSYE RAFAELA; GIMENES NANTES, WESLEY ARRUDA; SANTOS, FILIPE MARTINS; DE OLIVEIRA PORFIRIO, GRASIELA EDITH; BARROS-BATTESTI, DARCI MORAES; et al. Novel Ehrlichia and Hepatozoon genotypes in white-eared opossums (Didelphis albiventris) and associated ticks from Brazil. TICKS AND TICK-BORNE DISEASES, v. 13, n. 6, p. 10-pg., 2022-08-13. (19/26774-9, 19/04415-7, 19/15150-4, 18/02753-0)

SANTOS, FILIPE MARTINS; SANO, NAYARA YOSHIE; PERLES, LIVIA; ANDRE, MARCOS ROGERIO. Host-parasite relationship in urban environments: A network analysis of haemoparasite infections in Nasua nasua Linnaeus (South American coati). Medical and Veterinary Entomology, v. N/A, p. 8-pg., 2025-03-24. (19/15150-4, 20/12037-0, 18/02753-0)

BASSINI-SILVA, RICARDO; CASTRO-SANTIAGO, ANA CAROLINA; CALCHI, ANA CLAUDIA; PERLES, LIVIA; TAKATSU, JULIA CARVALHO; ALENCAR, ISABEL D. C. C.; OCHOA, RONALD; DOWLING, ASHLEY P. G.; WERTHER, KARIN; ANDRE, MARCOS ROGERIO; et al. Sleeping with the enemy: case reports of Ornithonyssus bursa (Berlese, 1888) (Mesostigmata: Macronyssidae) causing human dermatitis in Brazil. Parasitology Research, v. 121, n. 9, p. 9-pg., 2022-07-08. (21/06758-9, 18/24667-8, 19/15150-4, 21/02295-4, 20/07826-5, 20/11755-6, 19/19853-0, 17/01416-7)