Molecular detection and characterization of Anaplasmataceae and Bartonellaceae agents in free-ranging wild boars (Sus scrofa) in the State of São Paulo

• Grant number: 19/24726-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2020
• End date: February 28, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Marcos Rogério André
• Grantee: Matheus de Souza Santana
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

According to the World Health Organization, 60% of pathogens are zoonotic, which are responsible for the occurrence of 75% of emerging diseases; of these, 22.8% are carried by arthropod vectors. Anaplasmataceae and Bartonellaceae agents are, respectively, intracellular obligate and facultative, alpha-proteobacteria, Gram-negative, which have been detected in several mammalian species, including humans. Although wild boars are not native to Brazil, they have a wide geographical distribution and are responsible for crop destruction, they can act as reservoirs of pathogens and may favor the extinction of species by the competitive exclusion. There are few studies on the role of these animals as hosts for the above pathogens. The present study aims to verify, using molecular techniques, the occurrence and genetic diversity of Anaplasmataceae and Bartonellaceae agents in free-ranging wild boars in Brazil. To this purpose, 123 wild boar blood samples from the state of São Paulo and 148 ectoparasites (ticks and lice) samples collected from these animals will be analyzed. The blood and ectoparasites samples will be submitted to real-time and conventional PCR assays for Anaplasmataceae (based on groEL, dsb, vlpt, msp-2, groESL, gltA, omp-1 and sodB genes) and Bartonellaceae (based on nuoG, ribC, gltA, rpoB, pap-31, fstZ, ITS genes) agents. The phylogenetic positioning of the sequences obtained will be performed through the construction of dendrograms. Finally, the sequences alignments will be used to calculate the nucleotide diversity and the level of polymorphisms for the analysis of genetic diversity. The genealogy of the nucleotide sequences will be performed using the Splitstree program. Thus, this study will contribute to the understanding of the role of wild boars as hosts for pathogens of zoonotic importance. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTANA, MATHEUS DE SOUZA; HOPPE, ESTEVAM GUILHERME LUX; CARRARO, PAULO EDUARDO; CALCHI, ANA CLAUDIA; DE OLIVEIRA, LARYSSA BORGES; DO AMARAL, RENAN BRESSIANINI; MONGRUEL, ANNA CLAUDIA BAUMEL; MACHADO, DALIA MONIQUE RIBEIRO; BURGER, KARINA PAES; BARROS-BATESTTI, DARCI MORAES; et al. Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia, Anaplasma, and haemoplasmas. TRANSBOUNDARY AND EMERGING DISEASES, v. 69, n. 5, p. 24-pg., 2022-07-11. (19/26403-0, 20/12037-0, 19/24726-7, 20/07826-5, 18/02753-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTANA, Matheus de Souza. Detection and molecular characterization of Anaplasmataceae, Bartonellaceae, Coxiellaceae, Mycoplasmataceae, Rickettsiaceae, Babesiidae and Theileriidae agents in free-living wild boars in São Paulo State, Brazil: Molecular detection of vector-borne agents in wild boars (Sus scrofa) and associated ticks in Brazil, with evidence of possible new genotypes of Ehrlichia, Anaplasma and Hemoplasma. 2022. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.