Identification and pathogenicity ofNeophysopellaspecies associated with Asian grapevine leaf rust in Brazil

Autor(es)

Santos, Ricardo F. ^[1] ; Primiano, V, Isabela ; Amorim, Lilian ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Dept Plant Pathol & Nematol, BR-13418900 Piracicaba, SP - Brazil

^[2] Primiano, Isabela, V, Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Dept Plant Pathol & Nematol, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANT PATHOLOGY; v. 70, n. 1, p. 74-86, JAN 2021.
• Citações Web of Science: 0

Resumo

Asian grapevine leaf rust (AGLR) causes severe crop losses in Brazilian viticulture, mainly in latitudes <25 degrees S. The purpose of this study was to identify the pathogen(s) involved with AGLR in Brazil, based on phylogenetic and morphological analysis and pathogenicity tests. In total, 56 monouredinial isolates from six Brazilian states were identified using the internal transcribed spacer 2 and the large subunit rRNA gene D1/D2 regions. All 50 isolates from the south-central region were classified asNeophysopella tropicalis, and the other six isolates from the north-east region asNeophysopella meliosmae-myrianthae. This result provides evidence that two pathogen introductions from different sources may have occurred in the country. For both species, paraphyses were cylindrical, incurved, aseptate, and hyaline, while urediniospores were short-pedicellate, obovoid or obovoid-ellipsoid, with the wall colourless or pale yellowish, evenly echinulate. Representative isolates from both species caused typical AGLR symptoms onVitis vinifera'Merlot' andV.labrusca'Niagara Rosada'. Overall, regardless of theNeophysopellaspecies, isolates caused similar leaf disease severities. Higher disease severity was observed in Niagara Rosada (average of 40.3% of diseased leaf area) compared to Merlot (20.5%). This study reports, for the first time, the characterization ofNeophysopellaspecies associated with AGLR in Brazil. (AU)

• Processo FAPESP: 15/26108-8 - Agressividade de Phakopsora: histopatologia e epidemiologia comparativa das ferrugens da videira e da soja
• Beneficiário: Isabela Vescove Primiano
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/24003-9 - Epidemiologia, avaliação de danos e controle de doenças da videira
• Beneficiário: Lilian Amorim
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/21412-6 - Estrutura populacional e sensibilidade de Plasmopara viticola e Phakopsora euvitis a fungicidas no Brasil
• Beneficiário: Ricardo Feliciano dos Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado