Structural analysis of CACHE domain of the McpA chemoreceptor from Leptospira interrogans

Autor(es)

Santos, Jademilson C. ^[1] ; Vieira, Monica L. ^[2] ; Abendroth, Jan ^{[3, 4]} ; Lin, Tao ^[5] ; Staker, Bart L. ^{[6, 4]} ; Myler, Peter J. ^{[6, 4, 7, 8]} ; Nascimento, Ana Lucia T. O. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Desenvolvimento Vacinas, Ave Vital Brasil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[2] Univ Fed Minas Gerais UFMG, Inst Ciencias Biol ICB, Dept Microbiol, Belo Horizonte, MG - Brazil

^[3] UCB Pharma SA, 7869 NE Day Rd West, Bainbridge Isl, WA 98110 - USA

^[4] Seattle Struct Genom Ctr Infect Dis, Seattle, WA 98109 - USA

^[5] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 - USA

^[6] Seattle Childrens Res Inst, Seattle, WA 98109 - USA

^[7] Univ Washington, Dept Pediat, Dept Biomed Informat & Hlth Educ, Seattle, WA 98105 - USA

^[8] Univ Washington, Dept Global Hlth, Seattle, WA 98105 - USA

• Tipo de documento: Artigo Científico
• Fonte: Biochemical and Biophysical Research Communications; v. 533, n. 4, p. 1323-1329, DEC 17 2020.
• Citações Web of Science: 0

Resumo

Leptospira is a genus of spirochete bacteria highly motile that includes pathogenic species responsible to cause leptospirosis disease. Chemotaxis and motility are required for Leptospira infectivity, pathogenesis, and invasion of bacteria into the host. In prokaryotes, the most common chemoreceptors are methyl accepting chemotaxis proteins that have a role play to detect the chemical signals and move to a favorable environment for its survival. Here, we report the first crystal structure of CACHE domain of the methyl-accepting chemotaxis protein (McpA) of L. interrogans. The structural analysis showed that McpA adopts similar a/b architecture of several other bacteria chemoreceptors. We also found a typical dimerization interface that appears to be functionally crucial for signal transmission and chemotaxis. In addition to McpA structural analyses, we have identified homologous proteins and conservative functional regions using bioinformatics techniques. These results improve our understanding the relationship between chemoreceptor structures and functions of Leptospira species. (C) 2020 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 14/50981-0 - Busca de proteínas de superfície nas sequências do genoma da Leptospira interrogans: caracterização funcional e imunológica para o entendimento de mecanismos envolvidos na patogênese de bactéria
• Beneficiário: Ana Lucia Tabet Oller Do Nascimento
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/25167-6 - Caracterização estrutural de proteínas de superfície de Leptospira interrogans e termodinâmica da interação com componentes do hospedeiro
• Beneficiário: Jademilson Celestino dos Santos
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado