Acute renal denervation normalizes aortic function and decreases blood pressure in spontaneously hypertensive rats

Autor(es)

Dias Moreira, Nathalia Juocys ^{[1, 2]} ; dos Santos, Fernando ^[1] ; Moreira, Edson Dias ^[1] ; Farah, Daniela ^{[1, 2]} ; de Souza, Leandro Eziquiel ^[1] ; da Silva, Maikon Barbosa ^[1] ; Moraes-Silva, Ivana Cinthya ^[1] ; Lincevicius, Gisele Silverio ^{[1, 2]} ; Caldini, Elia Garcia ^[3] ; Costa Irigoyen, Maria Claudia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo InCor FMUSP, Fac Med, Inst Coracao, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, EPM, UNIFESP, Sao Paulo - Brazil

^[3] Univ Sao Paulo, HC, FMUSP, Dept Patol, Fac Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 DEC 11 2020.
• Citações Web of Science: 0

Resumo

Mechanisms involved in the acute responses to renal denervation (RDN) have yet to be fully understood. We assessed urinary volume, autonomic control and aorta vascular reactivity after acute RDN. Male normotensive Wistar rats and spontaneously hypertensive rats (SHR) were divided into normotensive+RDN (ND) or sham surgery (NS), and hypertensive+RDN (HD) or sham surgery (HS). Metabolic parameters and hemodynamic measurements were recorded 72h and 4 days after intervention, respectively. Aortic rings were studied 7 days post RDN in an isometric myograph. Concentration-response curves to phenylephrine, sodium nitroprusside and acetylcholine (10(-10)-10(-5) M) were performed. Two-way ANOVA was used for group comparisons and differences reported when p<0.05. Results are presented as mean<plus/minus>SEM. Urinary volume was 112% higher in HD vs. HS (HS=14.94 +/- 2.5 mL; HD=31.69 +/- 2.2 mL) and remained unchanged in normotensive rats. Systolic BP was lower in HD rats (HS=201 +/- 12 vs. HD=172 +/- 3 mmHg) without changes in normotensive group. HD group showed increased HF and LF modulation (HS=5.8 +/- 0.7 ms(2)vs. HD=13.4 +/- 1.4 ms(2); HS=3.5 +/- 0.7 ms(2)vs. HD=10.5 +/- 1.7 ms(2), respectively). RDN normalized vascular reactivity in HD rats and increased phenylephrine response in ND rats. Acute fall in BP induced by RDN is associated with increased urinary volume, which in turn may also have contributed to functional changes of the aorta. (AU)

• Processo FAPESP: 18/19006-2 - Associação entre biomarcadores séricos, funcionais e exames de imagem na disfunção diastólica e cognitiva
• Beneficiário: Maria Claudia Costa Irigoyen
• Modalidade de apoio: Auxílio à Pesquisa - Regular