Zinc phthalocyanine tetrasulfonate-loaded polyelectrolytic PLGA nanoparticles for photodynamic therapy applications

Autor(es)

Modesto Clementino de Toledo, Maria Cristina ^[1] ; Abreu, Alexandro da Silva ^[2] ; Carvalho, Janicy Arantes ^[2] ; Ribeiro Ambrosio, Jessica Aparecida ^[1] ; Godoy, Daniele da Silva ^[1] ; Santos Pinto, Bruna Cristina dos ^[1] ; Junior, Milton Beltrame ^[1] ; Simioni, Andreza Ribeiro ^[1]

Afiliação do(s) autor(es)

^[1] Vale Paraiba Univ, Organ Synth Lab, Res & Dev Inst IPD, Ave Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos - Brazil

^[2] Univ Sao Paulo, Ctr Nanotechnol & Tissue Engn Photobiol & Photome, Dept Chem, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 32, DEC 2020.
• Citações Web of Science: 0

Resumo

Background: Photodynamic Therapy (PDT) is a modality for the treatment of neoplastic tissues, which is based on the administration of a phototherapeutic agent and light irradiation at an appropriate wavelength, aiming to locate and destroy the target cell with the formation of reactive oxygen species. Nanoencapsulation technology presents itself as a tool for incorporation of bioactive substances aiming to improve their solubility in physiological environment, obtain a longer circulation time in the organism, administration of lower dosages and the minimization of side effects. The present work aimed at the development of poly (lactic acid-glycolic acid) (PLGA) nanoparticles coated with polyelectrolyte film layers for encapsulating zinc phthalocyanine tetrasulfonated (ZnPcSO4) as a bioactive substance model. Methods: PLGA nanoparticles were produced by the double emulsion/solvent evaporation technique and poly electrolytic coating was performed using polyalkylamine hydrochloride (PAH) as a weak polycation and poly (4-styrene sulfonate) (PSS) as a strong polyanion by layer-by-layer self-assembly technique (known as layer-bylayer-LbL). The nanoparticulate system was studied by scanning electron microscopy, steady-state, and their biological activity was evaluated using in vitro cancer cell lines by classical MTT assay. Results: The polyelectrolytic PLGA nanoparticles had an average diameter of 384.7 +/- 138.6 nm, restricted distribution size with a polydispersity index. The obvious change in zeta potential indicates successful alternation in polycation (PAH) and polyanion (PSS) deposition directly in PLGA nanoparticles. Scanning electron microscopy (SEM) analysis showed that the formed system had morphology spherical, typical of these release systems. The loading efficiency was 82.1 % +/- 1.2 %. The polyelectrolytic nanoparticles loaded with phthalocyanine maintained their photophysical behavior after encapsulation. Cell viability was determined, obtaining 90 % cell death. Conclusions: Therefore, the presented work depicts ZnPcSO4-loaded polyelectrolytic PLGA nanoparticles as a promise drug delivery system for phototherapeutic agent, which are thus expected to have superior therapeutic efficiency than free drug. (AU)

• Processo FAPESP: 18/18531-6 - Microcápsulas polieletrolíticas como modelo de sistema de liberação controlada para fármacos fotossensíveis
• Beneficiário: Andreza Ribeiro Simioni
• Modalidade de apoio: Auxílio à Pesquisa - Regular