Aptasensor based on screen-printed electrode for breast cancer detection in undiluted human serum

Autor(es)

Ferreira, Daisy C. ^[1] ; Batistuti, Marina R. ^[1] ; Bachour Junior, Bassam ^[1] ; Mulato, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Philosophy Sci & Letters, Dept Phys, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOELECTROCHEMISTRY; v. 137, FEB 2021.
• Citações Web of Science: 0

Resumo

Breast cancer remains one of the leading causes of women death. The development of more sensitive diagnostic tests, which could present a faster response, lower cost, and could promote early diagnosis would increase the chances of survival. This study reports the development and optimization of an electrochemical aptasensor for the detection of HER2 protein, a breast cancer biomarker. Two sensing platforms were developed on gold screen-printed electrodes. The first platform is composed of self-assembled monolayer (SAM) made from mixture of thiolated DNA aptamers specific for HER2 and 1-mercapto-6-hexanol (MCH), while the second one is a ternary SAM composed of the same aptamer and 1,6-hexanethiol (HDT). Both platforms were further passivated with MCH and blocked with bovine serum albumin. The biosensors were characterized using electrochemical impedance spectroscopy to detect the target protein from 1 pg/mL to 1 mu g/mL in phosphate buffered saline, diluted and undiluted human serum through charge transfer resistance value. The ternary SAM architecture shows a reduction of non-specific attachment to the electrode surface due to the HDT antifouling properties. In addition, this platform exhibits 172 pg/mL as limit of detection and a sensitivity of 4.12% per decade for undiluted serum compared with SAM architecture with the 179 pg/mL and 4.32% per decade, respectively. Electrochemical aptasensors are highly promising for medical diagnostic and ternary layers could improve the limit of detection. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 17/13161-3 - Biossensores eletroquímicos para detecção da proteína HER2 relacionada ao câncer de mama
• Beneficiário: Daisy Camargo Ferreira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/26133-7 - Detecção de alterações relacionadas ao câncer de mama através de sensores de carga ou de massa acoplados a monocamadas de DNA
• Beneficiário: Marina Ribeiro Batistuti Sawazaki
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/24201-6 - Plataformas Microeletrônicas para Biossensores Eletroquímicos, Piezoelétricos e FETs
• Beneficiário: Marcelo Mulato
• Modalidade de apoio: Auxílio à Pesquisa - Regular