Aptasensor based on screen-printed electrode for breast cancer detection in undiluted human serum

Author(s)

Ferreira, Daisy C. ^[1] ; Batistuti, Marina R. ^[1] ; Bachour Junior, Bassam ^[1] ; Mulato, Marcelo ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Philosophy Sci & Letters, Dept Phys, BR-14040901 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: BIOELECTROCHEMISTRY; v. 137, FEB 2021.
• Web of Science Citations: 0

Abstract

Breast cancer remains one of the leading causes of women death. The development of more sensitive diagnostic tests, which could present a faster response, lower cost, and could promote early diagnosis would increase the chances of survival. This study reports the development and optimization of an electrochemical aptasensor for the detection of HER2 protein, a breast cancer biomarker. Two sensing platforms were developed on gold screen-printed electrodes. The first platform is composed of self-assembled monolayer (SAM) made from mixture of thiolated DNA aptamers specific for HER2 and 1-mercapto-6-hexanol (MCH), while the second one is a ternary SAM composed of the same aptamer and 1,6-hexanethiol (HDT). Both platforms were further passivated with MCH and blocked with bovine serum albumin. The biosensors were characterized using electrochemical impedance spectroscopy to detect the target protein from 1 pg/mL to 1 mu g/mL in phosphate buffered saline, diluted and undiluted human serum through charge transfer resistance value. The ternary SAM architecture shows a reduction of non-specific attachment to the electrode surface due to the HDT antifouling properties. In addition, this platform exhibits 172 pg/mL as limit of detection and a sensitivity of 4.12% per decade for undiluted serum compared with SAM architecture with the 179 pg/mL and 4.32% per decade, respectively. Electrochemical aptasensors are highly promising for medical diagnostic and ternary layers could improve the limit of detection. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 17/13161-3 - Electrochemical biosensors for detection of HER2 protein linked to breast cancer
• Grantee: Daisy Camargo Ferreira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/26133-7 - Detection of changes related with breast cancer using charge sensors or mass sensors coupled to DNA monolayers
• Grantee: Marina Ribeiro Batistuti Sawazaki
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/24201-6 - Microelectronic Platforms for Electrochemical, Piezoelectric and FETs Biosensors
• Grantee: Marcelo Mulato
• Support Opportunities: Regular Research Grants