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Detection of changes related with breast cancer using charge sensors or mass sensors coupled to DNA monolayers

Grant number: 13/26133-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2014
Effective date (End): May 31, 2017
Field of knowledge:Engineering - Electrical Engineering
Principal Investigator:Marcelo Mulato
Grantee:Marina Ribeiro Batistuti
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:12/22820-7 - The sensitive label free detection of disease markers using electrochemical immittance, AP.TEM
Associated scholarship(s):15/14404-1 - Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers, BE.EP.DR   15/14403-5 - Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers, BE.EP.DR

Abstract

Unidimensional structures of aromatic crystals coupling have as property transporting charge. It has been proposed that the DNA structure might have similar behavior due to its structural similarity with these crystals. Your role as a promising material in electronic devices immediately generated conflicting results, ranging from insulating to superconducting. Barton and his colleagues were crucial to elucidate the mechanisms of transport depending on the size and sequence of the DNA strand. From this understanding was possible to initiate the development of biosensors. The DNA adhered to a transducer forms a self-assembled monolayer. Similarly, the pairing of two complementary sequences allows the construction of sensors with high selectivity and the oxidation of guanine bases also allows the use of electrochemical techniques to the study, characterization of this type of biosensor. Based on the application of SAM in DNA sensors, we propose in this project the development of charge sensor and mass sensor through the field effect transistor with an extended port (also the English extended gate field effect transistor - EGFET) and microbalance quartz crystal. Our focus will be to detect different sequences related to changes bases due to breast cancer.

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BATISTUTI, Marina Ribeiro. Detecção de alterações relacionadas ao câncer de mama através de sensores de carga ou de massa acoplados a monocamadas de DNA. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto Ribeirão Preto.

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