Insulin Modulates Myogenesis and Muscle Atrophy Resulting From Skin Scald Burn in Young Male Rats

Autor(es)

Quintana, Hananiah Tardivo ^[1] ; de Araujo Baptista, Vivianne Izabelle ^[1] ; Lazzarin, Mariana Cruz ^[1] ; Moreira Antunes, Hanna Karen ^[1] ; Le Sueur-Maluf, Luciana ^[1] ; Machado de Oliveira, Camila Aparecida ^[1] ; de Oliveira, Flavia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biociencias, Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SURGICAL RESEARCH; v. 257, p. 56-68, JAN 2021.
• Citações Web of Science: 0

Resumo

Background: Burn injuries (BIs) due to scalding are one of the most common accidents among children. BIs greater than 40% of total body surface area are considered extensive and result in local and systemic response. We sought to assess morphological and myogenic mechanisms through both shortand long-term intensive insulin therapies that affect the skeletal muscle after extensive skin BI in young rats. Materials and methods: Wistar rats aged 21 d were distributed into four groups: control (C), control with insulin (C + I), scald burn injury (SI), and SI with insulin (SI + I). The SI groups were submitted to a 45% total body surface area burn, and the C + I and SI + I groups received insulin (5 UI/Kg/d) for 4 or 14 d. Glucose tolerance and the homeostatic model assessment of insulin resistance index were determined. Gastrocnemius muscles were analyzed for histopathological, morphometric, and immunohistochemical myogenic parameters (Pax7, MyoD, and MyoG); in addition, the expression of genes related to muscle atrophy (MuRF1 and MAFbx) and its regulation (IGF-1) were also assessed. Results: Short-term treatment with insulin favored muscle regeneration by primary myogenesis and decreased muscle atrophy in animals with BIs, whereas the long-term treatment modulated myogenesis by increasing the MyoD protein. Both treatments improved histopathological parameters and secondary myogenesis by increasing the MyoG protein. Conclusions: Treatment with insulin benefits myogenic parameters during regeneration and modulates MuRF1, an important mediator of muscle atrophy. Published by Elsevier Inc. (AU)

• Processo FAPESP: 14/11458-0 - Aspectos morfoquantitativos e moleculares do músculo esquelético de ratos submetidos ao tratamento com insulina após lesão térmica
• Beneficiário: Hananiah Tardivo Quintana
• Modalidade de apoio: Bolsas no Brasil - Doutorado