| Full text | |
| Author(s): |
Quintana, Hananiah Tardivo
[1]
;
de Araujo Baptista, Vivianne Izabelle
[1]
;
Lazzarin, Mariana Cruz
[1]
;
Moreira Antunes, Hanna Karen
[1]
;
Le Sueur-Maluf, Luciana
[1]
;
Machado de Oliveira, Camila Aparecida
[1]
;
de Oliveira, Flavia
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Biociencias, Santos, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | JOURNAL OF SURGICAL RESEARCH; v. 257, p. 56-68, JAN 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Background: Burn injuries (BIs) due to scalding are one of the most common accidents among children. BIs greater than 40% of total body surface area are considered extensive and result in local and systemic response. We sought to assess morphological and myogenic mechanisms through both shortand long-term intensive insulin therapies that affect the skeletal muscle after extensive skin BI in young rats. Materials and methods: Wistar rats aged 21 d were distributed into four groups: control (C), control with insulin (C + I), scald burn injury (SI), and SI with insulin (SI + I). The SI groups were submitted to a 45% total body surface area burn, and the C + I and SI + I groups received insulin (5 UI/Kg/d) for 4 or 14 d. Glucose tolerance and the homeostatic model assessment of insulin resistance index were determined. Gastrocnemius muscles were analyzed for histopathological, morphometric, and immunohistochemical myogenic parameters (Pax7, MyoD, and MyoG); in addition, the expression of genes related to muscle atrophy (MuRF1 and MAFbx) and its regulation (IGF-1) were also assessed. Results: Short-term treatment with insulin favored muscle regeneration by primary myogenesis and decreased muscle atrophy in animals with BIs, whereas the long-term treatment modulated myogenesis by increasing the MyoD protein. Both treatments improved histopathological parameters and secondary myogenesis by increasing the MyoG protein. Conclusions: Treatment with insulin benefits myogenic parameters during regeneration and modulates MuRF1, an important mediator of muscle atrophy. Published by Elsevier Inc. (AU) | |
| FAPESP's process: | 14/11458-0 - Morphoquantitative and molecular aspects of skeletal muscle of rats submitted to treatment with insulin after burn injury |
| Grantee: | Hananiah Tardivo Quintana |
| Support Opportunities: | Scholarships in Brazil - Doctorate |