Chromosomal segments may explain the antibody response cooperation for canine leishmaniasis pathogenesis

Autor(es):Mostrar menos -

Batista, Luis F. S. ^[1] ; Torrecilha, Rafaela B. P. ^[2] ; Silva, Rafaela B. ^[3] ; Utsunomiya, Yuri T. ^[4] ; Silva, Thais B. F. ^[1] ; Tomokane, Thaise Y. ^[1] ; Pacheco, Acacio D. ^[5] ; Bosco, Anelise M. ^[5] ; Paulan, Silvana C. ^[4] ; Rossi, Claudio N. ^[6] ; Costa, Gustavo N. O. ^[2] ; Marcondes, Mary ^[5] ; Ciarlini, Paulo C. ^[5] ; Nunes, Caris M. ^[4] ; Matta, Vania L. R. ^[1] ; Laurenti, Marcia D. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Lab Patol Doencas Infecciosas, BR-01246903 Sao Paulo - Brazil

^[2] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, BR-14884900 Jaboticabal, SP - Brazil

^[3] Univ Salvador, Escola Saude, BR-41720200 Salvador, BA - Brazil

^[4] Univ Estadual Paulista, Fac Med Vet Aracatuba, Dept Apoio Prod & Saude Anim, BR-16015050 Aracatuba, SP - Brazil

^[5] Univ Estadual Paulista, Fac Med Vet, Dept Clin Cirurgia & Reprod Anim, BR-16015050 Aracatuba, SP - Brazil

^[6] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 288, DEC 2020.
• Citações Web of Science: 0

Resumo

Visceral leishmaniasis (VL) is marked by hyperactivation of a humoral response secreting high quantity of immunoglobulins (Igs) that are inaccessible to intracellular parasites. Here we investigated the contributions of the antibody response to the canine leishmaniasis pathogenesis. Using correlation and genome-wide association analysis, we investigated the relationship of anti-Leishmania infantum immunoglobulin classes levels with parasite burden, clinical response, renal/hepatic biochemical, and oxidative stress markers in dogs from endemic areas of VL. Immunoglobulin G (IgG) and IgA were positively correlated with parasite burden on lymph node and blood. Increased IgG, IgA and IgE levels were associated with severe canine leishmaniasis (CanL) whereas IgM was elevated in uninfected exposed dogs. Correlations of IgM, IgG and IgA with creatinine, urea, AST and ALT levels in the serum were suggested an involvement of those Igs with renal and hepatic changes. The correlogram of oxidative radicals and antioxidants revealed a likely relationship of IgM, IgG and IgA with oxidative stress and lipid pemxidation in the blood, suggested as mechanisms mediating tissue damage and CanL worsening. The gene mapping on chromosomal segments associated with the quantitative variation of immunoglobulin classes identified genetic signatures involved with reactive oxygen species generation, phagolysosome maturation and rupture, free iron availability, Thl/Th2 differenciation and, immunoglobulin clearance. The findings demonstrated the roles of the antibody response as resistance or susceptibility markers and mediators of CanL pathogenesis. In addition we pinpointed candidate genes as potential targets for the therapy against the damage caused by exacerbated antibody response and parasitism in VL. (AU)

• Processo FAPESP: 12/05847-9 - Avaliação clínica, imunológica e suas correlações com marcadores genéticos na leishmaniose visceral canina.
• Beneficiário: Luís Fábio da Silva Batista
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/01095-8 - Utilização de sequenciamento de nova geração para o mapeamento fino de regiões cromossômicas que afetam o perímetro escrotal de touros Nelore
• Beneficiário: Yuri Tani Utsunomiya
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/50285-9 - Avaliação clinica,imunologica e suas correlações com marcadores genéticos na leishmaniose visceral canina
• Beneficiário: Marcia Dalastra Laurenti
• Modalidade de apoio: Auxílio à Pesquisa - Regular