Development and validation of quantitative analytical method for 50 drugs of antidepressants, benzodiazepines and opioids in oral fluid samples by liquid chromatography-tandem mass spectrometry

Autor(es)

Furiozo Arantes, Ana Carolina ^{[1, 2]} ; da Cunha, Kelly Francisco ^{[1, 2]} ; Cardoso, Marilia Santoro ^{[1, 2]} ; Oliveira, Karina Diniz ^{[1, 2]} ; Costa, Jose Luiz ^{[3, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, BR-13083859 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Campinas Poison Control Ctr, BR-13083859 Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083871 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Forensic Toxicology; v. 39, n. 1 DEC 2020.
• Citações Web of Science: 0

Resumo

Purpose We developed and validated a method for quantitative analysis of 50 psychoactive substances and metabolites (antidepressants, benzodiazepines and opioids) in oral fluid samples using simple liquid-liquid extraction procedure followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Method Oral fluid samples were collected using Quantisal (TM) device and extracted by liquid-liquid extraction with 1.0 mL of methyl tert-butyl ether and then analyzed using LC-MS/MS. Results The method attended method validation criteria, with limits of quantification as low as 0.5 and 1.0 ng/mL, and linearity between 0.5-50.0 ng/mL for antidepressants, 0.5-25.0 ng/mL for benzodiazepines and 1.0-50.0 ng/mL to opioids. During method validation, bias and imprecision values were not greater than 16 and 20%, respectively. Ionization suppression/enhancement bias results were not greater than 25%. No evidence of carryover was observed. Sample stability studies showed that almost all analytes were stable at 25 degrees C for 3 days and at 4 degrees C for 7 days. Freeze-thaw cycles stability showed that most antidepressants and opioids were stable under these conditions. Autosampler stability study showed that all analytes were stable for 24 h, except for nitrazepam and 7-aminoclonazepam. Thirty-eight authentic oral fluid samples were analyzed; 36.8% of the samples were positive for 2 drugs. Citalopram was the most common drug found, followed by venlafaxine. Conclusions The method was validated according to international recommendations for the 50 analytes, showing low limits of quantification, good imprecision and bias values, using simple liquid-liquid extraction, and was successfully applied to authentic oral fluid samples analysis. (AU)

• Processo FAPESP: 18/00432-1 - A Toxicologia das Novas Substâncias Psicoativas (NSP): epidemiologia do consumo através da análise de amostras de cabelo e fluido oral
• Beneficiário: José Luiz da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/11849-0 - A toxicologia das Novas Substâncias Psicoativas (NSP): epidemiologia do consumo através da análise de amostras de fluido oral e caracterização de seu metabolismo in vitro
• Beneficiário: Kelly Francisco da Cunha
• Modalidade de apoio: Bolsas no Brasil - Doutorado