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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development and validation of quantitative analytical method for 50 drugs of antidepressants, benzodiazepines and opioids in oral fluid samples by liquid chromatography-tandem mass spectrometry

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Author(s):
Furiozo Arantes, Ana Carolina [1, 2] ; da Cunha, Kelly Francisco [1, 2] ; Cardoso, Marilia Santoro [1, 2] ; Oliveira, Karina Diniz [1, 2] ; Costa, Jose Luiz [3, 2]
Total Authors: 5
Affiliation:
[1] Univ Estadual Campinas, Fac Med Sci, BR-13083859 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Campinas Poison Control Ctr, BR-13083859 Campinas, SP - Brazil
[3] Univ Estadual Campinas, Fac Pharmaceut Sci, BR-13083871 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Forensic Toxicology; v. 39, n. 1 DEC 2020.
Web of Science Citations: 0
Abstract

Purpose We developed and validated a method for quantitative analysis of 50 psychoactive substances and metabolites (antidepressants, benzodiazepines and opioids) in oral fluid samples using simple liquid-liquid extraction procedure followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Method Oral fluid samples were collected using Quantisal (TM) device and extracted by liquid-liquid extraction with 1.0 mL of methyl tert-butyl ether and then analyzed using LC-MS/MS. Results The method attended method validation criteria, with limits of quantification as low as 0.5 and 1.0 ng/mL, and linearity between 0.5-50.0 ng/mL for antidepressants, 0.5-25.0 ng/mL for benzodiazepines and 1.0-50.0 ng/mL to opioids. During method validation, bias and imprecision values were not greater than 16 and 20%, respectively. Ionization suppression/enhancement bias results were not greater than 25%. No evidence of carryover was observed. Sample stability studies showed that almost all analytes were stable at 25 degrees C for 3 days and at 4 degrees C for 7 days. Freeze-thaw cycles stability showed that most antidepressants and opioids were stable under these conditions. Autosampler stability study showed that all analytes were stable for 24 h, except for nitrazepam and 7-aminoclonazepam. Thirty-eight authentic oral fluid samples were analyzed; 36.8% of the samples were positive for 2 drugs. Citalopram was the most common drug found, followed by venlafaxine. Conclusions The method was validated according to international recommendations for the 50 analytes, showing low limits of quantification, good imprecision and bias values, using simple liquid-liquid extraction, and was successfully applied to authentic oral fluid samples analysis. (AU)

FAPESP's process: 18/00432-1 - The Toxicology of New Psychoactive Substances (NSP): epidemiology of consumption by the analysis of hair and oral fluid samples
Grantee:José Luiz da Costa
Support Opportunities: Regular Research Grants
FAPESP's process: 18/11849-0 - The toxicology of New Psychoactive Substances (NSP): epidemiology of consumption by oral fluid samples and characterization of their in vitro metabolism
Grantee:Kelly Francisco da Cunha
Support Opportunities: Scholarships in Brazil - Doctorate