Development of an analytical method for the determination of methylphenidate, ethylphenidate and ritalinic acid in oral fluid samples using liquid chromatography-tandem mass spectrometry

• Grant number: 19/02866-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2020
• Effective date (End): January 31, 2021
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: José Luiz da Costa
• Grantee: Kauê de Oliveira Chinaglia
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications, AP.TEM

Abstract

Methylphenidate (MFD) is an active principle known in Brazil under the trade name Ritalin®, a substance structurally related to amphetamines. It is one of the most consumed stimulants in the world, being used mainly for the treatment of Attention Deficit Hyperactivity Disorder (ADHD). However, due to psychostimulatory effects, many university students who do not present criteria that justify the use of the drug and its analogue methylphenidate (EFD), use them as a means to improve the yield of the studies, since the drug also increases the ability to concentrate, attention and alertness in healthy people. The use of MFD to improve cognitive performance was called "pharmacological cognitive enhancement" and drew the attention of several countries such as Canada, the United States and Brazil. Although there are several cases in the world of abusive use of MFD and EFD, there are few methods related to quantification using oral fluid and campaigns to prevent the use of these compounds as drugs of abuse. The objective of this study is to develop a quantitative method for the analysis of methylphenidate, methylphenidate analogue and ritualistic acid metabolite in oral fluid using liquid chromatography coupled to sequential mass spectrometry (LC-MS / MS). The method developed will be used for the analysis of oral fluid samples collected at university festivities in the city of Campinas-SP.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHINAGLIA, KAUE DE OLIVEIRA; ARANTES, ANA CAROLINA FURIOZO; DA CUNHA, KELLY FRANCISCO; DE CAMPOS, EDUARDO GERALDO; KAHL, JULIA MARTINELLI MAGALHAES; RODRIGUES, LEONARDO COSTALONGA; COSTA, JOSE LUIZ. Development of analytical method for the determination of methylphenidate, the analog ethylphenidate and their metabolite ritalinic acid in oral fluid samples by micro-QuEChERS and liquid chromatography-tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1205, p. 7-pg., 2022-06-15. (19/02866-1, 20/10809-5)