Association between genetic polymorphisms in the promoter region of the defensin beta 1 gene and persistent apical periodontitis

Autor(es)

Antunes, L. S. ^{[1, 2]} ; Carvalho, L. ^{[1, 3]} ; Petean, I. B. F. ^[4] ; Antunes, L. A. ^{[1, 2]} ; Freitas, J. V. ^[5] ; Salles, A. G. ^{[1, 3]} ; Olej, B. ^[1] ; Oliveira, D. S. B. ^[6] ; Kuchler, E. C. ^{[5, 7]} ; Sousa-Neto, M. D. ^[4]

Afiliação do(s) autor(es)

^[1] Fluminense Fed Univ, Clin Res Unit, Niteroi, RJ - Brazil

^[2] Fluminense Fed Univ, Sch Dent Nova Friburgo, Dept Specif Format, Rua Doutor Silvio Henrique Braune, 22 Ctr, BR-28625650 Nova Friburgo, RJ - Brazil

^[3] Fluminense Fed Univ, Sch Dent, Postgrad Program, Niteroi, RJ - Brazil

^[4] Univ Sao Paulo, Sch Dent Ribeirao Preto, Restorat Dent Dept, Ribeirao Preto - Brazil

^[5] Univ Posit, Sch Hlth & Biol Sci, Curitiba, Parana - Brazil

^[6] Univ Fed Alfenas, Sch Dent, Dept Clin & Surg, Alfenas, MG - Brazil

^[7] Univ Sao Paulo, Sch Dent Ribeirao Preto, Pediat Dent Dept, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 54, n. 1, p. 38-45, JAN 2021.
• Citações Web of Science: 0

Resumo

Aim To evaluate the association between the promoter region of defensin beta 1 (DEFB1) genetic polymorphisms and persistent apical periodontitis (PAP) in Brazilian patients. Methodology Seventy-three patients with post-treatment PAP (PAP group) and 89 patients with root filled teeth with healed and healthy periradicular tissues (healed group) were included (all teeth had apical periodontitis lesions at the beginning of the treatment). Patients who had undergone at least 1 year of follow-up after root canal treatment were recalled, and their genomic DNA was extracted from saliva. Two single nucleotide polymorphisms (SNPs) inDEFB1at the g. -52G>A (rs1799946) and g. -20G>A (rs11362) positions were analysed using real-time polymerase chain reaction. The chi-squared test was performed, and the odds ratios were calculated using Epi Info 3.5.2. Logistic regression analysis in the codominant model, using the time of follow-up as a variable, was used to evaluate the SNP-SNP interaction. All tests were performed with an established alpha of 0.05 (P = 0.05). Results For the rs11362 polymorphism in the codominant and recessive models, patients who carried two copies of the T allele had a significantly lower risk of developing PAP (P = 0.040 andP = 0.031, respectively). For the rs1799946 polymorphism inDEFB1in the codominant and recessive models, carrying one copy of the T allele significantly increased the risk of developing PAP (P = 0.007 andP = 0.031, respectively). In the logistic regression, both polymorphisms were associated with PAP as well as the SNP-SNP interaction (P < 0.0001). Conclusions Polymorphisms inDEFB1genes were associated with the development of post-treatment persistent apical periodontitis. (AU)

• Processo FAPESP: 18/21130-3 - Aspectos moleculares da resposta do hospedeiro na periodontite apical persistente
• Beneficiário: Manoel Damiao de Sousa Neto
• Modalidade de apoio: Auxílio à Pesquisa - Regular