Hydroxyapatite-CoFe2O4 Magnetic Nanoparticle Composites for Industrial Enzyme Immobilization, Use, and Recovery

Autor(es)

Coutinho, Thamara C. ^{[1, 2]} ; Malafatti, Joao O. D. ^{[1, 3]} ; Paris, Elaine C. ^{[1, 3]} ; Tardioli, Paulo W. ^[2] ; Farinas, Cristiane S. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Embrapa Instrumentat, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Grad Program Chem, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED NANO MATERIALS; v. 3, n. 12, p. 12334-12345, DEC 24 2020.
• Citações Web of Science: 0

Resumo

Hydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzyme immobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes beta-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70-100% and recovered activities of 78-100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field, demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the beta-glucosidase biocatalyst, which could be reused 10 times, maintaining around 70% of its initial activity. (AU)

• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático