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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hydroxyapatite-CoFe2O4 Magnetic Nanoparticle Composites for Industrial Enzyme Immobilization, Use, and Recovery

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Author(s):
Coutinho, Thamara C. [1, 2] ; Malafatti, Joao O. D. [1, 3] ; Paris, Elaine C. [1, 3] ; Tardioli, Paulo W. [2] ; Farinas, Cristiane S. [1, 2]
Total Authors: 5
Affiliation:
[1] Embrapa Instrumentat, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Grad Program Chem, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ACS APPLIED NANO MATERIALS; v. 3, n. 12, p. 12334-12345, DEC 24 2020.
Web of Science Citations: 0
Abstract

Hydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzyme immobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes beta-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70-100% and recovered activities of 78-100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field, demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the beta-glucosidase biocatalyst, which could be reused 10 times, maintaining around 70% of its initial activity. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants