IgG4-positive plasma cells are more often detected in chronic periapical lesions arising from permanent rather than primary teeth

Autor(es)

Polanco, X. B. J. ^[1] ; Bertasso, A. S. ^[1] ; Silveira, H. A. ^{[2, 3]} ; Yamamoto de Almeida, L. ^[2] ; Almeida, L. K. Y. ^[1] ; da Silva, R. A. B. ^[1] ; da Silva, L. A. B. ^[1] ; de Rossi, A. ^[1] ; Nelson-Filho, P. ^[1] ; Leon, J. E. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Dent Sch FORP USP, Dept Pediat Dent, Ribeirao Preto - Brazil

^[2] Sao Paulo State Univ UNESP, Araraquara Dent Sch, Dept Diag & Surg, Oral Med, Araraquara, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Dent Sch FORP USP, Dept Stomatol Publ Oral Hlth & Forens Dent, Oral Pathol, BR-14040904 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 54, n. 5 JAN 2021.
• Citações Web of Science: 0

Resumo

Aim To characterize plasma cell subsets in chronic periapical lesions affecting permanent and primary teeth. Methodology Only chronic periapical lesions without root canal treatment were selected. Twenty-one radicular cysts and 7 periapical granulomas affecting permanent teeth and 19 radicular cysts and 4 periapical granulomas affecting primary teeth were assessed for immunoglobulin (Ig) light chain (kappa and lambda), Ig heavy chain (IgG, IgG4, IgA, IgM and IgD) and plasma cell immunohistochemical markers (MUM1/IRF4, EMA and CD138). The data acquired were analysed by Student's t test, Mann-Whitney U, Friedman test followed by Dunn's multiple comparison test and Spearman's rank correlation. Results All cases were polyclonal (having similar kappa/lambda light chain ratios). IgG was most abundant compared to other Ig heavy chains (all, P < 0.001); like Ig light chains, but unlike IgA, there was greater expression of IgG in the primary compared to the permanent dentition, for both radicular cysts (P < 0.001) and periapical granulomas (P = 0.53). Notably, IgG4 expression was greater in the permanent than the primary dentition, for both radicular cyst (P < 0.05) and periapical granuloma (P = 0.65). IgM and IgD expression was scarce and variable, whereas plasma cell populations were detected efficiently through EMA, CD138 and MUM1/IRF4 markers, the latter being more sensitive in both dentitions. Conclusions There were slight variations in the Ig light and heavy chain profiles in chronic periapical lesions when comparing the permanent and primary dentitions. The ability of IgG4+ plasma cell infiltration to modulate inflammatory responses in chronic periapical lesions arising from permanent as opposed to primary teeth should be considered in future studies. (AU)

• Processo FAPESP: 16/02713-2 - Efeitos da terapia com erlotinib e gefitinib em associação ao ácido all-trans retinóico e trióxido de arsênico na leucemia promielocítica aguda: estudo in vitro, ex vivo e em modelo experimental murino.
• Beneficiário: Luciana Yamamoto de Almeida
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/11419-0 - Correlação clinicopatológica do grau de diferenciação, expressão de tetraspaninas, agentes virais e proliferação celular em uma ampla série de carcinomas espinocelulares da região de cabeça e pescoço.
• Beneficiário: Jorge Esquiche León
• Modalidade de apoio: Auxílio à Pesquisa - Regular