Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics

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Tahmasebi, Roozbeh ^{[1, 2]} ; Luchs, Adriana ^[3] ; Tardy, Kaelan ^[2] ; Hefford, Philip Michael ^[4] ; Tinker, Rory J. ^[5] ; Eilami, Owrang ^[6] ; de Padua Milagres, Flavio Augusto ^{[7, 8, 9]} ; Brustulin, Rafael ^{[7, 8, 9]} ; Rodrigues Teles, Maria da Aparecida ^{[7, 9]} ; Morais, Vanessa dos Santos ^[2] ; Valente Moreira, Carlos Henrique ^[3] ; Buccheri, Renata ^[2] ; Lima Araujo, Emerson Luiz ^[10] ; Villanova, Fabiola ^[11] ; Deng, Xutao ^{[12, 13]} ; Sabino, Ester Cerdeira ^{[1, 2]} ; Delwart, Eric ^{[12, 13]} ; Leal, Elcio ^[11] ; da Costa, Antonio Charlys ^[2]

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^[1] Univ Sao Paulo, Polytech Sch, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil

^[3] Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, Sao Paulo - Brazil

^[4] Univ Hosp Plymouth NHS Trust, Plymouth, Devon - England

^[5] Univ Manchester, Fac Biol Med & Hlth, Manchester M13 9PL, Lancs - England

^[6] Yasuj Univ Med Sci, Determinants Hlth Res Ctr, Sch Med Social, Yasuj - Iran

^[7] Secretary Hlth Tocantins, Tocantins - Brazil

^[8] Publ Hlth Lab Tocantins State LACEN TO, Tocantins - Brazil

^[9] Fed Univ Tocantins, Inst Biol Sci, Tocantins - Brazil

^[10] Minist Hlth CGLAB DAEVS SVS MS, Gen Coordinat Publ Hlth Labs, Hlth Surveillance Secretariat, Strateg Articulat Dept, Brasilia, DF - Brazil

^[11] Fed Univ Para, Inst Biol Sci, Belem, Para - Brazil

^[12] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA - USA

^[13] Vitalant Res Inst, San Francisco, CA - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF GENERAL VIROLOGY; v. 101, n. 12, p. 1280-1288, 2020.
• Citações Web of Science: 1

Resumo

Human enteric adenovirus species F (HAdV-F) is one of the most common pathogens responsible for acute gastroenteritis worldwide. Brazil is a country with continental dimensions where continuous multiregional surveillance is vital to establish a more complete picture of the epidemiology of HAdV-F. The aim of the current study was to investigate the molecular epidemiology of HAdV-F using full-genome data in rural and low-income urban areas in northern Brazil. This will allow a genetic comparison between Brazilian and global HAdV-F strains. The frequency of HAdV-F infections in patients with gastroenteritis and molecular typing of positive samples within this period was also analysed. A total of 251 stool samples collected between 2010 and 2016 from patients with acute gastroenteritis were screened for HAdV-F using next-generation sequencing techniques. HAdV-F infection was detected in 57.8% (145/251) of samples. A total of 137 positive samples belonged to HAdV-F41 and 7 to HAdV-F40. HAdV-F40/41 dual infection was found in one sample. Detection rates did not vary significantly according to the year. Single HAdV-F infections were detected in 21.9% (55/251) of samples and mixed infections in 37.4% (94/251), with RVA/HAdV-F being the most frequent association (21.5%; 54/251). Genetic analysis indicated that the HAdV-F strains circulating in Brazil were closely related to worldwide strains, and the existence of some temporal order was not observed. This is the first large-scale HAdV-F study in Brazil in which whole-genome data and DNA sequence analyses were used to characterize HAdV-F strains. Expanding the viral genome database could improve overall genotyping success and assist the National Center for Biotechnology Information (NCBI)/GenBank in standardizing the HAdV genome records by providing a large set of annotated HAdV-F genomes. (AU)

• Processo FAPESP: 19/21706-5 - Metagenômica viral de mosquitos capturados no Estado de São Paulo
• Beneficiário: Vanessa dos Santos Morais
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 17/00021-9 - Metagenômica viral da Dengue, Chikungunya e Zika: acompanhar, explicar e prever a transmissão e distribuição espaço-temporal no Brasil
• Beneficiário: Antonio Charlys da Costa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado