Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics

Full text
Author(s):
Show less -
Tahmasebi, Roozbeh [1, 2] ; Luchs, Adriana [3] ; Tardy, Kaelan [2] ; Hefford, Philip Michael [4] ; Tinker, Rory J. [5] ; Eilami, Owrang [6] ; de Padua Milagres, Flavio Augusto [7, 8, 9] ; Brustulin, Rafael [7, 8, 9] ; Rodrigues Teles, Maria da Aparecida [7, 9] ; Morais, Vanessa dos Santos [2] ; Valente Moreira, Carlos Henrique [3] ; Buccheri, Renata [2] ; Lima Araujo, Emerson Luiz [10] ; Villanova, Fabiola [11] ; Deng, Xutao [12, 13] ; Sabino, Ester Cerdeira [1, 2] ; Delwart, Eric [12, 13] ; Leal, Elcio [11] ; da Costa, Antonio Charlys [2]
Total Authors: 19
Affiliation:
Show less -
[1] Univ Sao Paulo, Polytech Sch, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Trop Med, Sao Paulo - Brazil
[3] Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, Sao Paulo - Brazil
[4] Univ Hosp Plymouth NHS Trust, Plymouth, Devon - England
[5] Univ Manchester, Fac Biol Med & Hlth, Manchester M13 9PL, Lancs - England
[6] Yasuj Univ Med Sci, Determinants Hlth Res Ctr, Sch Med Social, Yasuj - Iran
[7] Secretary Hlth Tocantins, Tocantins - Brazil
[8] Publ Hlth Lab Tocantins State LACEN TO, Tocantins - Brazil
[9] Fed Univ Tocantins, Inst Biol Sci, Tocantins - Brazil
[10] Minist Hlth CGLAB DAEVS SVS MS, Gen Coordinat Publ Hlth Labs, Hlth Surveillance Secretariat, Strateg Articulat Dept, Brasilia, DF - Brazil
[11] Fed Univ Para, Inst Biol Sci, Belem, Para - Brazil
[12] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA - USA
[13] Vitalant Res Inst, San Francisco, CA - USA
Total Affiliations: 13
Document type: Journal article
Source: JOURNAL OF GENERAL VIROLOGY; v. 101, n. 12, p. 1280-1288, 2020.
Web of Science Citations: 1
Abstract

Human enteric adenovirus species F (HAdV-F) is one of the most common pathogens responsible for acute gastroenteritis worldwide. Brazil is a country with continental dimensions where continuous multiregional surveillance is vital to establish a more complete picture of the epidemiology of HAdV-F. The aim of the current study was to investigate the molecular epidemiology of HAdV-F using full-genome data in rural and low-income urban areas in northern Brazil. This will allow a genetic comparison between Brazilian and global HAdV-F strains. The frequency of HAdV-F infections in patients with gastroenteritis and molecular typing of positive samples within this period was also analysed. A total of 251 stool samples collected between 2010 and 2016 from patients with acute gastroenteritis were screened for HAdV-F using next-generation sequencing techniques. HAdV-F infection was detected in 57.8% (145/251) of samples. A total of 137 positive samples belonged to HAdV-F41 and 7 to HAdV-F40. HAdV-F40/41 dual infection was found in one sample. Detection rates did not vary significantly according to the year. Single HAdV-F infections were detected in 21.9% (55/251) of samples and mixed infections in 37.4% (94/251), with RVA/HAdV-F being the most frequent association (21.5%; 54/251). Genetic analysis indicated that the HAdV-F strains circulating in Brazil were closely related to worldwide strains, and the existence of some temporal order was not observed. This is the first large-scale HAdV-F study in Brazil in which whole-genome data and DNA sequence analyses were used to characterize HAdV-F strains. Expanding the viral genome database could improve overall genotyping success and assist the National Center for Biotechnology Information (NCBI)/GenBank in standardizing the HAdV genome records by providing a large set of annotated HAdV-F genomes. (AU)

FAPESP's process: 19/21706-5 - Viral metagenomics of mosquitoes captured in the State of São Paulo
Grantee:Vanessa dos Santos Morais
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
Grantee:Antonio Charlys da Costa
Support Opportunities: Scholarships in Brazil - Post-Doctoral