Advanced search
Start date
Betweenand

Investigating the evoltution of animal rotavirus strains infecting humans

Grant number: 15/12944-9
Support type:Regular Research Grants
Duration: March 01, 2016 - February 28, 2018
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Adriana Luchs
Grantee:Adriana Luchs
Home Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Assoc. researchers:Maria Do Carmo Sampaio Tavares Timenetsky

Abstract

Group A rotaviruses (RVA) are the main cause of acute viral gastroenteritis in both humans and animal. Global surveillance of circulating RVA in the human population has revealed the presence of several uncommon genotypes, including Brazil. Many of these have been found in domestic animals, and phylogenetic analysis suggests that they arose in the human population through zoonotic transmission. The distribution and evolution of human RVA strains is important for vaccine development and effectiveness. In 2006 an attenuated monovalent vaccine was included in the Brazilian National Immunization Program, preventing severe RVA gastroenteritis, and inducing significant reduction in the frequency of RVA detection in children with gastroenteritis. In setting where RVA vaccine coverage is high, such as in Brazil, vaccine pressure could select for replacement of common strains (similar to those included in RVA vaccines) with uncommon strains, some of which could be generated by reassortment between human and animal RVA. The Enteric Diseases Laboratory (NDE) of Adolfo Lutz Institute is a macro-regional reference center for RVA surveillance, Ministry of Health (MS)-Brazil. Convenient stool samples from patients with acute gastroenteritis were sent to the NDE for viral diagnosis. The fecal specimens are investigated for RVA by commercial ELISA kit and genotyped by RT-PCR for the two outer capsid proteins: VP4 (P genotype) and VP7 (G genotype). The detection of unusual/rare G and P genotypes association strongly suggest interspecies transmission. Since 2008, the RVA classification system based on molecular characterization of the 11 genes (genomic constellation) have been used to study viral evolution, and provided an excellent means of deciphering the true origin of unconventional strains, especially those derived from interspecies transmission and subsequent adaptation to human host. Tracking virus evolution and gaining an understanding of the role that animal RVA strains play is important for continued development of vaccine strategies and genotyping surveillance. The aim of this study is investigate the dynamic between human and animal RVA strains using complete genome sequencing of RVA strains displaying rare/unusual G (VP7) and P (VP4) genotypes combinations, following phylogetic analysis. (AU)

Articles published in Agência FAPESP about the research grant
Virus that infects pigs in China is found in human patient in Brazil 

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA COSTA, ANTONIO CHARLYS; LEAL, ELCIO; GILL, DANIELLE; DE PADUA MILAGRES, FLAVIO AUGUSTO; KOMNINAKIS, SHIRLEY VASCONCELOS; BRUSTULIN, RAFAEL; RODRIGUES TELES, MARIA DA APARECIDA; BRITO SAYAO LOBATO, MARCIA CRISTINA ALVES; DAS CHAGAS, ROGERIO TOGISAKI; NEVES DOS SANTOS ABRAO, MARIA DE FATIMA; DE DEUS ALVES SOARES, CASSIA VITORIA; DENG, XUTAO; DELWART, ERIC; SABINO, ESTER CERDEIRA; LUCHS, ADRIANA. Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil. VIRUS GENES, v. 55, n. 3, p. 332-338, JUN 2019. Web of Science Citations: 0.
LUCHS, ADRIANA; DA COSTA, ANTONIO CHARLYS; CILLI, AUDREY; VASCONCELOS KOMNINAKIS, SHIRLEY CAVALCANTE; COMPAGNOLI CARMONA, RITA DE CASSIA; BOEN, LAIS; MORILLO, SIMONE GUADAGNUCCI; SABINO, ESTER CERDEIRA; SAMPAIO TAVARES TIMENETSKY, MARIA DO CARMO. Spread of the emerging equine-like G3P[8] DS-1-like genetic backbone rotavirus strain in Brazil and identification of potential genetic variants. JOURNAL OF GENERAL VIROLOGY, v. 100, n. 1, p. 7-25, JAN 2019. Web of Science Citations: 1.
RIBEIRO, GEOVANI DE OLIVEIRA; LUCHS, ADRIANA; DE PADUA MILAGRES, FLAVIO AUGUSTO; KOMNINAKIS, SHIRLEY VASCONCELOS; GILL, DANIELLE ELISE; BRITO SAYAO LOBATO, MARCIA CRISTINA ALVES; BRUSTULIN, RAFAEL; DAS CHAGAS, ROGERIO TOGISAKI; NEVES DOS SANTOS ABRAO, MARIA DE FATIMA; DE DEUS ALVES SOARES, CASSIA VITORIA; WITKIN, STEVEN S.; VILLANOVA, FABIOLA; DENG, XUTAO; SABINO, ESTER CERDEIRA; DELWART, ERIC; DA COSTA, ANTONIO CHARLYS; LEAL, ELCIO. Detection and Characterization of Enterovirus B73 from a Child in Brazil. Viruses-Basel, v. 11, n. 1 JAN 2019. Web of Science Citations: 0.
DA COSTA, ANTONIO CHARLYS; LUCHS, ADRIANA; DE PADUA MILAGRES, FLAVIO AUGUSTO; KOMNINAKIS, SHIRLEY VASCONCELOS; GILL, DANIELLE ELISE; BRITO SAYAO LOBATO, MARCIA CRISTINA ALVES; BRUSTULIN, RAFAEL; DAS CHAGAS, ROGERIO TOGISAKI; NEVES DOS SANTOS ABRAO, MARIA DE FATIMA; DE DEUS ALVES SOARES, CASSIA VITORIA; DENG, XUTAO; SABINO, ESTER CERDEIRA; DELWART, ERIC; LEAL, ELCIO. Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus. Viruses-Basel, v. 10, n. 10 OCT 2018. Web of Science Citations: 0.
DA COSTA, ANTONIO CHARLYS; LUCHS, ADRIANA; DE PADUA MILAGRES, FLAVIO AUGUSTO; KOMNINAKIS, SHIRLEY VASCONCELOS; GILL, DANIELLE ELISE; BRITO SAYAO LOBATO, MARCIA CRISTINA ALVES; BRUSTULIN, RAFAEL; DAS CHAGAS, ROGERIO TOGISAKI; NEVES DOS SANTOS ABRAO, MARIA DE FATIMA; DE DEUS ALVES SOARES, CASSIA VITORIA; DENG, XUTAO; SABINO, ESTER CERDEIRA; DELWART, ERIC; LEAL, ELCIO. Near full length genome of a recombinant (E/D) cosavirus strain from a rural area in the central region of Brazil. SCIENTIFIC REPORTS, v. 8, AUG 17 2018. Web of Science Citations: 0.
LUCHS, ADRIANA; LEAL, ELCIO; KOMNINAKIS, SHIRLEY VASCONCELOS; DE PADUA MILAGRES, FLAVIO AUGUSTO; BRUSTULIN, RAFAEL; RODRIGUES TELES, MARIA DA APARECIDA; GILL, DANIELLE ELISE; DENG, XUTAO; DELWART, ERIC; SABINO, ESTER CERDEIRA; DA COSTA, ANTONIO CHARLYS. Wuhan large pig roundworm virus identified in human feces in Brazil. VIRUS GENES, v. 54, n. 3, p. 470-473, JUN 2018. Web of Science Citations: 1.
ARANHA WATANABE, ARIPUANA SAKURADA; LUCHS, ADRIANA; LEAL, ELCIO; DE PADUA MILAGRES, FLAVIO AUGUSTO; KOMNINAKIS, SHIRLEY VASCONCELOS; GILL, DANIELLE ELISE; BRITO SAYAO LOBATO, MARCIA CRISTINA ALVES; BRUSTULIN, RAFAEL; DAS CHAGAS, ROGERIO TOGISAKI; NEVES DOS SANTOS ABRAO, MARIA DE FATIMA; DE DEUS ALVES SOARES, CASSIA VITORIA; DENG, XUTAO; SABINO, ESTER CERDEIRA; DELWART, ERIC; DA COSTA, ANTONIO CHARLYS. Complete Genome Sequences of Six Human Bocavirus Strains from Patients with Acute Gastroenteritis in the North Region of Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 17 APR 2018. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.