Differential expression of antioxidant system genes in honey bee (Apis mellifera L.) caste development mitigates ROS-mediated oxidative damage in queen larvae

Autor(es)

Santos, Douglas Elias ^[1] ; Souza, Anderson de Oliveira ^[2] ; Tiberio, Gustavo Jacomini ^[1] ; Alberici, Luciane Carla ^[2] ; Hartfelder, Klaus ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias BioMol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 43, n. 4 2020.
• Citações Web of Science: 0

Resumo

The expression of morphological differences between the castes of social bees is triggered by dietary regimes that differentially activate nutrient-sensing pathways and the endocrine system, resulting in differential gene expression during larval development. In the honey bee, Apis mellifera, mitochondrial activity in the larval fat body has been postulated as a link that integrates nutrient-sensing via hypoxia signaling. To understand regulatory mechanisms in this link, we measured reactive oxygen species (ROS) levels, oxidative damage to proteins, the cellular redox environment, and the expression of genes encoding antioxidant factors in the fat body of queen and worker larvae. Despite higher mean H2O2 levels in queens, there were no differences in ROS-mediated protein carboxylation levels between the two castes. This can be explained by their higher expression of antioxidant genes (MnSOD, CuZnSOD, catalase, and Gst1) and the lower ratio between reduced and oxidized glutathione (GSH/GSSG). In worker larvae, the GSG/GSSH ratio is elevated and antioxidant gene expression is delayed. Hence, the higher ROS production resulting from the higher respiratory metabolism in queen larvae is effectively counterbalanced by the up-regulation of antioxidant genes, avoiding oxidative damage. In contrast, the delay in antioxidant gene expression in worker larvae may explain their endogenous hypoxia response. (AU)

• Processo FAPESP: 13/24733-7 - O metabolismo oxidativo na diferenciação de castas em abelhas melíferas: número e estrutura mitocondrial e expressão de genes indicadores de funcionalidade
• Beneficiário: Douglas Elias Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/26418-2 - Estudo da via de sinalização de AMPK: potenciais alvos regulados e diferenças na expressão genica durante o controle do desenvolvimento de castas em abelhas melíferas
• Beneficiário: Douglas Elias Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/09128-0 - Bases moleculares da quebra da síndrome trade-off entre reprodução e longevidade no organismo modelo de insetos sociais, Apis mellifera
• Beneficiário: Klaus Hartmann Hartfelder
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/03171-5 - Análise causal do desenvolvimento de Apis mellifera: genes reguladores e redes hierárquicas de expressão gênica na especificação de tecidos e órgãos
• Beneficiário: Zilá Luz Paulino Simões
• Modalidade de apoio: Auxílio à Pesquisa - Temático