Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential expression of antioxidant system genes in honey bee (Apis mellifera L.) caste development mitigates ROS-mediated oxidative damage in queen larvae

Full text
Author(s):
Santos, Douglas Elias [1] ; Souza, Anderson de Oliveira [2] ; Tiberio, Gustavo Jacomini [1] ; Alberici, Luciane Carla [2] ; Hartfelder, Klaus [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias BioMol, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: GENETICS AND MOLECULAR BIOLOGY; v. 43, n. 4 2020.
Web of Science Citations: 0
Abstract

The expression of morphological differences between the castes of social bees is triggered by dietary regimes that differentially activate nutrient-sensing pathways and the endocrine system, resulting in differential gene expression during larval development. In the honey bee, Apis mellifera, mitochondrial activity in the larval fat body has been postulated as a link that integrates nutrient-sensing via hypoxia signaling. To understand regulatory mechanisms in this link, we measured reactive oxygen species (ROS) levels, oxidative damage to proteins, the cellular redox environment, and the expression of genes encoding antioxidant factors in the fat body of queen and worker larvae. Despite higher mean H2O2 levels in queens, there were no differences in ROS-mediated protein carboxylation levels between the two castes. This can be explained by their higher expression of antioxidant genes (MnSOD, CuZnSOD, catalase, and Gst1) and the lower ratio between reduced and oxidized glutathione (GSH/GSSG). In worker larvae, the GSG/GSSH ratio is elevated and antioxidant gene expression is delayed. Hence, the higher ROS production resulting from the higher respiratory metabolism in queen larvae is effectively counterbalanced by the up-regulation of antioxidant genes, avoiding oxidative damage. In contrast, the delay in antioxidant gene expression in worker larvae may explain their endogenous hypoxia response. (AU)

FAPESP's process: 13/24733-7 - Oxidative metabolism in caste differentiation in honeybees: number and structure of gene expression and mitochondrial functional indicators
Grantee:Douglas Elias Santos
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/26418-2 - AMPK signaling pathway: potential regulated targets and effects on gene expression during control of caste development in honeybees
Grantee:Douglas Elias Santos
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/09128-0 - Molecular basis of the disruption in the reproduction-longevity trade-off syndrome in the social insect model organism Apis mellifera
Grantee:Klaus Hartmann Hartfelder
Support Opportunities: Regular Research Grants
FAPESP's process: 11/03171-5 - Causal analysis of Apis mellifera development: regulatory genes and hierachical networks of gene expression in the specification of tissue and organs
Grantee:Zilá Luz Paulino Simões
Support Opportunities: Research Projects - Thematic Grants