Protective effects of luteolin on the venous endothelium

Autor(es)

Reis Assuncao, Henrique Charlanti ^[1] ; Coelho Cruz, Yan Milen ^[1] ; Bertolino, Jessica Silva ^[1] ; Tamborelli Garcia, Raphael Caio ^[1] ; Fernandes, Liliam ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Ciencias Farmaceut, Campus Diadema, BR-09913030 Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular and Cellular Biochemistry; v. 476, n. 4 JAN 2021.
• Citações Web of Science: 0

Resumo

Luteolin is a flavonoid with antioxidant properties already demonstrated in studies related to inflammation, tumor, and cardiovascular processes; however, there are no available information regarding its antioxidant effects at the venous endothelial site. We investigated the effects of luteolin (10, 20, and 50 mu mol/L) in cultures of rat venous endothelial cells. Nitric oxide (NO) and reactive oxygen species (ROS) were analyzed by fluorimetry; 3-nitrotyrosine (3-NT) residues were evaluated by immunofluorescence, and prostacyclin (PGI(2)) release was investigated by colorimetry. Intracellular NO levels were significantly enhanced after 10 min of luteolin incubation, with a parallel decrease in ROS generation. These results were accompanied by a significant reduction in the expression of 3-NT residues and enhanced PGI(2) rates. Therefore, luteolin is effective in reducing ROS thereby improving NO availability in venous endothelial cells. Besides, luteolin-induced decrease in 3-NT residues may correlate with the enhancement in endothelial PGI(2) bioavailability. These findings suggest the future application of this flavonoid as a protective agent by improving endothelial function in several circulatory disorders related to venous insufficiency. (AU)

• Processo FAPESP: 17/22028-5 - Efeitos da luteolina sobre a geração endotelial de espécies reativas de oxigênio induzida por Angiotensina II.
• Beneficiário: Liliam Fernandes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/21834-8 - Exposição à cetamina, ao etanol e à associação de ambas as substâncias: avaliação da neurotoxicidade em células SH-SY5Y
• Beneficiário: Raphael Caio Tamborelli Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Regular