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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protective effects of luteolin on the venous endothelium

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Author(s):
Reis Assuncao, Henrique Charlanti [1] ; Coelho Cruz, Yan Milen [1] ; Bertolino, Jessica Silva [1] ; Tamborelli Garcia, Raphael Caio [1] ; Fernandes, Liliam [1]
Total Authors: 5
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Ciencias Farmaceut, Campus Diadema, BR-09913030 Diadema, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Molecular and Cellular Biochemistry; v. 476, n. 4 JAN 2021.
Web of Science Citations: 0
Abstract

Luteolin is a flavonoid with antioxidant properties already demonstrated in studies related to inflammation, tumor, and cardiovascular processes; however, there are no available information regarding its antioxidant effects at the venous endothelial site. We investigated the effects of luteolin (10, 20, and 50 mu mol/L) in cultures of rat venous endothelial cells. Nitric oxide (NO) and reactive oxygen species (ROS) were analyzed by fluorimetry; 3-nitrotyrosine (3-NT) residues were evaluated by immunofluorescence, and prostacyclin (PGI(2)) release was investigated by colorimetry. Intracellular NO levels were significantly enhanced after 10 min of luteolin incubation, with a parallel decrease in ROS generation. These results were accompanied by a significant reduction in the expression of 3-NT residues and enhanced PGI(2) rates. Therefore, luteolin is effective in reducing ROS thereby improving NO availability in venous endothelial cells. Besides, luteolin-induced decrease in 3-NT residues may correlate with the enhancement in endothelial PGI(2) bioavailability. These findings suggest the future application of this flavonoid as a protective agent by improving endothelial function in several circulatory disorders related to venous insufficiency. (AU)

FAPESP's process: 17/22028-5 - Effects of luteolin on the endothelial generation of reactive oxygen species induced by Angiotensin II.
Grantee:Liliam Fernandes
Support Opportunities: Regular Research Grants
FAPESP's process: 17/21834-8 - Exposure to ketamine, ethanol and ketamine-ethanol combination: neurotoxicity evaluation in SH-SY5Y neural cells
Grantee:Raphael Caio Tamborelli Garcia
Support Opportunities: Regular Research Grants