Detection of Giardia and Cryptosporidium in environmental matrices with immunomagnetic separation: two or three acid dissociations

Autor(es)

Andreoli, Fernando Cesar ^[1] ; Sabogal-Paz, Lyda Patricia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Hydraul & Sanitat, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Parasitology Research; v. 120, n. 2 JAN 2021.
• Citações Web of Science: 0

Resumo

This study evaluated the technology of detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in environmental matrices obtained after water treatment on a bench scale. Calcium carbonate flocculation with immunomagnetic separation was the selected method to quantify the protozoa, and the importance of the number of acid dissociations in the immunomagnetic separation was assessed. When adding the third acid dissociation, an increase of 71% +/- 6 in floated residue and 31.9% +/- 28.7 in filter backwash water in cyst recovery was observed, while in oocyst recovery, a non-significant increase was detected. In the filtered water, this increased dissociation was important in the protozoa recovery with increases greater than 33%. The results showed that there is a strong interaction of these target organisms with the magnetic microspheres, since protozoa were still recovered in the third acid dissociation and some of them were still adhered to the magnetic microspheres. (AU)

• Processo FAPESP: 12/50522-0 - Contaminação ambiental pelos protozoários Giardia spp. e Cryptosporidium spp. e por Ascaris suum: desafios de detecção, remoção e inativação das formas infectantes
• Beneficiário: Regina Maura Bueno Franco
• Modalidade de apoio: Auxílio à Pesquisa - Temático