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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detection of Giardia and Cryptosporidium in environmental matrices with immunomagnetic separation: two or three acid dissociations

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Author(s):
Andreoli, Fernando Cesar [1] ; Sabogal-Paz, Lyda Patricia [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Hydraul & Sanitat, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Parasitology Research; v. 120, n. 2 JAN 2021.
Web of Science Citations: 0
Abstract

This study evaluated the technology of detection of Giardia spp. cysts and Cryptosporidium spp. oocysts in environmental matrices obtained after water treatment on a bench scale. Calcium carbonate flocculation with immunomagnetic separation was the selected method to quantify the protozoa, and the importance of the number of acid dissociations in the immunomagnetic separation was assessed. When adding the third acid dissociation, an increase of 71% +/- 6 in floated residue and 31.9% +/- 28.7 in filter backwash water in cyst recovery was observed, while in oocyst recovery, a non-significant increase was detected. In the filtered water, this increased dissociation was important in the protozoa recovery with increases greater than 33%. The results showed that there is a strong interaction of these target organisms with the magnetic microspheres, since protozoa were still recovered in the third acid dissociation and some of them were still adhered to the magnetic microspheres. (AU)

FAPESP's process: 12/50522-0 - Environmental contamination by protozoa Giardia spp. and Cryptosporidium spp. and Ascaris suum: challenges of detection, removal and inactivation of infective forms
Grantee:Regina Maura Bueno Franco
Support Opportunities: Research Projects - Thematic Grants