Production of monoclonal antibodies for the detection of enteropathogens in clinical and environmental specimens

• Grant number: 00/05834-7
• Support Opportunities: Research Projects - Thematic Grants
• Duration: July 01, 2001 - February 28, 2007
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Elizabeth Natal de Gaspari
• Grantee: Elizabeth Natal de Gaspari
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The ability to detect the presence of pathogenic organisms in clinical specimens, food and water is extremely importat to reduce and/or prevent case of foodborne illness. Culture methods for bacterial detection are often lengthy, therefore rapid, sensitive, specific and simple methods of detection are needed. Rapid testing can provice processors with better quality assurance prorams, which in turn reduces the risk to consumers. If the co-agglutination technique can be optimized, it could be very useful to public health agencies to investigate, diagnose, and prevent foodborne illness outbreaks, Monoclonal antibody reagents formatted into rapid test systems (e.g. latex agglutionatio, ELISA, immunofluorescence, and immunomagnetic separation), can provide public health agencies with necessary diagnostic tolls to reduce the impact of food and water borne diseases. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BELO‚ E.F.T.; FERRAZ‚ A.S.; COUTINHO‚ L.M.C.C.; OLIVEIRA‚ A.P.; CARMO‚ A.M.S.; TUNES‚ C.F.; FERREIRA‚ T.; ITO‚ A.Y.; MACHADO‚ M.S.F.; FRANCO‚ D.D.L.; et al. Production of monoclonal antibodies against Neisseria meningitidis using popliteal lymph nodes and in vivo/in vitro immunization: prevalence study of new monoclonal antibodies in greater São Paulo‚ Brazil. HYBRIDOMA, v. 26, n. 5, p. 302-310, 2007. (00/05834-7)

ITO‚ A.Y.; NÉRI‚ S.; MACHADO‚ M.S.S.; TUNES‚ C.F.; DE GASPARI‚ E.N.. Homologous prime-boost strategy in neonate mice using< i> Neisseria lactamica. Vaccine, v. 27, n. 25, p. 3422-3428, 2009. (00/05834-7)

FERREIRA, TATIANE; DE GASPARI, ELIZABETH. The Design of New Adjuvants for Mucosal Immunity to Neisseria meningitidis B in Nasally Primed Neonatal Mice for Adult Immune Response. SCIENTIFIC WORLD JOURNAL, 2012. (00/05834-7, 08/54542-0)