Non-enzymatic lactose molecularly imprinted sensor based on disposable graphite paper electrode

Autor(es)

da Silva, Jose Luiz ^[1] ; Buffon, Edervaldo ^[1] ; Beluomini, Maisa Azevedo ^[1] ; Pradela-Filho, Lauro Antonio ^[2] ; Gouveia Araujo, Diele Aparecida ^[2] ; Santos, Andre Luiz ^[2] ; Takeuchi, Rgina Massako ^[2] ; Stradiotto, Nelson Ramos ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Chem, Analyt Chem Dept, 55 Prof Francisco Degni St, BR-14800060 Araraquara, SP - Brazil

^[2] Univ Fed Uberlandia, Inst Exact & Nat Sci Pontal, 1600 20 St, BR-38304402 Ituiutaba, MG - Brazil

^[3] Sao Paulo State Univ UNESP, Bioenergy Res Inst IPBEN, 55 Prof Francisco Degni St, BR-14800060 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Analytica Chimica Acta; v. 1143, p. 53-64, JAN 25 2021.
• Citações Web of Science: 3

Resumo

Lactose (LAC) is a disaccharide - major sugar, present in milk and dairy products. LAC content is an important indicator of milk quality and abnormalities in food industries, as well as in human and animal health. The present study reports the development of an innovative imprinted voltammetric sensor for sensitive detection of LAC. The sensor was constructed using electropolymerized pyrrole (Py) molecularly imprinted polymer (MIP) on graphite paper electrode (PE). The MIP film was constructed through the electrosynthesis of polypyrrole (PPy) in the presence of LAC (template molecule) on PE (PPy/PE). To optimize the detection conditions, several factors affecting the PPy/PE sensor performance were assessed by multivariate methods (Plackett-Burman design and central composite design). Under optimized conditions, the proposed analytical method was applied for LAC detection in whole and LAC-free milks, where it demonstrated high sensitivity and selectivity, with two dynamic linear ranges of concentration (1.0-10 nmol L-1 and 25-125 nmol L-1) and a detection limit of 0.88 nmol L-1. The MIP sensor showed selective molecular recognition for LAC in the presence of structurally related molecules. The proposed PPy/PE sensor exhibited good stability, as well as excellent reproducibility and repeatability. Based on the results obtained, the PPy/PE is found to be highly promising for sensitive detection of LAC. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 19/13818-8 - Desenvolvimento de sensores descartáveis baseados em óxido de grafeno 3D, nanopartículas metálicas e polímeros molecularmente impressos para determinação de ácidos fenólicos em resíduos da fruticultura
• Beneficiário: Edervaldo Buffon
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/25329-6 - Desenvolvimento de novos métodos analíticos para avaliação da qualidade de bioquerosene de aviação
• Beneficiário: Nelson Ramos Stradiotto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/12131-6 - Sensores eletroquímicos baseados em eletrodos impressos modificados com nanoporos metálicos e monitorados por rede sem fio para determinação de flavonóides nos resíduos da indústria cítrica
• Beneficiário: Maísa Azevedo Beluomini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado