Phenotypic detection of quorum sensing inhibition inPseudomonas aeruginosapyoverdine and swarming by volatile organic products

Autor(es)

Ramirez-Rueda, Roman Y. ^{[1, 2, 3]} ; Salvador, Marcos J. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, PPG BTPB, Dept Plant Biol, Postal Box 6109, BR-13083970 Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Inst Biol, PPG BV, Postal Box 6109, BR-13083970 Campinas, SP - Brazil

^[3] Pedag & Technol Univ Colombia, Fac Hlth Sci, Postal Box 0387437173, Calle 24 5-63, Tunja - Colombia

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 15, n. 12, p. 1147-1156, AUG 2020.
• Citações Web of Science: 0

Resumo

Aim:To determine phenotypically the anti quorum-sensing (QS) activity of 30 volatile organic products (VOPs) through the inhibition of swarming motility and pyoverdine production inPseudomonas aeruginosa.Materials \& methods:Twenty-four essential oils and six small volatile organic compounds randomly selected were screened for their anti-QS activity by violacein inhibition onChromobacterium violaceum. The VOPs with positive results were subsequently evaluated for swarming motility and pyoverdine production onP. aeruginosadetermining the colony diameter and fluorescence under UV light, respectively.Results:Fifty percent of VOPs tested showed strong violacein inhibition, 40% presented anti-swarming activity and 33% inhibited pyoverdine production.Conclusion:Our data demonstrate that VOPs have a great potential to inhibit virulence factors mediated by QS inP. aeruginosa (AU)

• Processo FAPESP: 15/03726-8 - Estudo químico e biológico integrado para a busca de substâncias bioativas em espécies vegetais* e desenvolvimento de procedimentos analíticos. *(Amaranthaceae, Gesneriaceae, Myrtaceae e Annonaceae)
• Beneficiário: Marcos José Salvador
• Modalidade de apoio: Auxílio à Pesquisa - Regular