Extensively drug-resistant IMP-16-producing Pseudomonas monteilii isolated from cerebrospinal fluid

Autor(es)

Ballaben, Anelise Stella ^[1] ; Galetti, Renata ^[1] ; Andrade, Leonardo Neves ^[1] ; Ferreira, Joseane Cristina ^[1] ; Garcia, Doroti de Oliveira ^[2] ; Doi, Yohei ^{[3, 4]} ; Costa Darini, Ana Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Fammceut Ribeirao Preto, Ribeirao Preto - Brazil

^[2] Inst Adolfo Lutz Marilia, Marilia, SP - Brazil

^[3] Univ Pittsburgh, Sch Med, Div Infect Dis, Pittsburgh, PA - USA

^[4] Univ Pittsburgh, Ctr Innovat Antimicrobial Therapy, Sch Med, Pittsburgh, PA - USA

• Tipo de documento: Artigo Científico
• Fonte: INFECTION GENETICS AND EVOLUTION; v. 87, JAN 2021.
• Citações Web of Science: 0

Resumo

IMP-1-producing Pseudomonas aeruginosa was first reported in Japan and since then, bacteria with this metallo-beta-lactamase have been detected worldwide. Pseudomonas monteilii (part of P. panda group) were considered an environmental pathogen with low virulence potential; however, multidrug-resistant and carbapenem-resistant P. monteilii have emerged. The present study reports the draft sequence of an extensively drug-resistant IMP16-producing P. monteilii 597/14 isolated from cerebrospinal fluid in 2014. The sequencing data revealed bla(IMP-)(16) as a gene cassette on class 1 integon, In1738 characterized in this study. Furthermore, the resistome of Pm597/14 consisted of 7 resistance genes (aadA1b, strA, strB, aacA4, bla(IMP-16), bla(OXA-2), sull) and diverse virulence determinants involved in the adherence, LPS, antiphagocytosis, iron uptake and mercuric resistance. Although different virulence determinants were found in this study, using Gaileria mellonella infection model, Pm597/14 did not kill any larvae between 7 days post-infection. P. monteilii isolates have been reported from clinical and environmental sources, carrying different MBL genes showing its potential role as their reservoir. (AU)

• Processo FAPESP: 14/14494-8 - Epidemiologia molecular de bactérias gram-negativas e genética da resistência a antibióticos
• Beneficiário: Ana Lúcia da Costa Darini
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/11728-0 - Sequenciamento completo de plasmídeos carreando genes de resistência a antibióticos em bactérias de interesse e análise do genoma de linhagens de Pseudomonas aeruginosa produtoras de SPM-1 e de KPC-2 por sequenciamento em larga escala
• Beneficiário: Renata Galetti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/23484-9 - Estudo molecular de bactérias gram-negativas produtoras de beta-lactamases isoladas de pacientes com suspeita de meningite na região de Ribeirão Preto
• Beneficiário: Anelise Stella Ballaben
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto