Extensively drug-resistant IMP-16-producing Pseudomonas monteilii isolated from cerebrospinal fluid

Author(s)

Ballaben, Anelise Stella ^[1] ; Galetti, Renata ^[1] ; Andrade, Leonardo Neves ^[1] ; Ferreira, Joseane Cristina ^[1] ; Garcia, Doroti de Oliveira ^[2] ; Doi, Yohei ^{[3, 4]} ; Costa Darini, Ana Lucia ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Fammceut Ribeirao Preto, Ribeirao Preto - Brazil

^[2] Inst Adolfo Lutz Marilia, Marilia, SP - Brazil

^[3] Univ Pittsburgh, Sch Med, Div Infect Dis, Pittsburgh, PA - USA

^[4] Univ Pittsburgh, Ctr Innovat Antimicrobial Therapy, Sch Med, Pittsburgh, PA - USA

• Document type: Journal article
• Source: INFECTION GENETICS AND EVOLUTION; v. 87, JAN 2021.
• Web of Science Citations: 0

Abstract

IMP-1-producing Pseudomonas aeruginosa was first reported in Japan and since then, bacteria with this metallo-beta-lactamase have been detected worldwide. Pseudomonas monteilii (part of P. panda group) were considered an environmental pathogen with low virulence potential; however, multidrug-resistant and carbapenem-resistant P. monteilii have emerged. The present study reports the draft sequence of an extensively drug-resistant IMP16-producing P. monteilii 597/14 isolated from cerebrospinal fluid in 2014. The sequencing data revealed bla(IMP-)(16) as a gene cassette on class 1 integon, In1738 characterized in this study. Furthermore, the resistome of Pm597/14 consisted of 7 resistance genes (aadA1b, strA, strB, aacA4, bla(IMP-16), bla(OXA-2), sull) and diverse virulence determinants involved in the adherence, LPS, antiphagocytosis, iron uptake and mercuric resistance. Although different virulence determinants were found in this study, using Gaileria mellonella infection model, Pm597/14 did not kill any larvae between 7 days post-infection. P. monteilii isolates have been reported from clinical and environmental sources, carrying different MBL genes showing its potential role as their reservoir. (AU)

• FAPESP's process: 14/14494-8 - Molecular epidemiology of gram-negative bacteria and genetic of antibiotic resistance
• Grantee: Ana Lúcia da Costa Darini
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/11728-0 - Complete sequencing of plasmids carrying antibiotic resistance genes in bacteria of interest and genome analysis of Pseudomonas aeruginosa-producing SPM-1 and KPC-2 lines by large-scale sequencing
• Grantee: Renata Galetti
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/23484-9 - Molecular study of gram-negative beta-lactamase producing bacteria isolated from patients with suspected meningitis in the Ribeirão Preto region
• Grantee: Anelise Stella Ballaben
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)